ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Affinity chromatography
;
Anhydrotrypsin
;
C-terminal Arg- or Lys-containing peptides
;
Diol silica
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Anhydrotrypsin (AHT), a catalytically inert derivative of trypsin in which the active site serine residue was converted to dehydroalanine residue by chemical modification, was immobilized onto diol silica through the activation with trifluoroethanesulfonyl chloride, and an AHT-diol-silica column was used for high-performance affinity chromatography separation of peptides containing arginine or lysine at their C-termini from the others. Improved separation in terms of speed was accomplished.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02328507
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