ISSN:
1432-1912
Keywords:
Affinity spectrum
;
Equilibrium binding isotherms
;
Linear programming
;
Likelihood function
;
Multiple receptor systems
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary For equilibrium binding isotherms of radioreceptor assays, the affinity spectrum is defined as a plot of the number of binding sites against their corresponding dissociation constants. A numerical procedure for direct calculation of affinity spectra from untransformed binding data is presented and illustrated with experimental values. The advantage of the new method in comparison to non-linear regression analysis is the fact that no starting values and mathematical models have to be supplied and that statistical assessment of the results is straightforward from a detailed graphical display of a likelihood function. Affinity spectra thus show directly all information formerly obtained by means of both graphical plots and regression analysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00500763
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