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  • Gonadotropin secretion  (2)
  • African catfish (Clarias lazera)  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Isolation of gonadotrops from the pituitary of the African catfish, Clarias lazera (1984)
    Springer
    Cell & tissue research 236 (1984), S. 669-675 
    Details
    ISSN: 1432-0878
    Keywords: Gonadotrops ; Isolation ; Cell culture ; Gonadotropin secretion ; African catfish (Clarias lazera)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dispersed pituitary cells from male African catfish, Clarias lazera, were fractionated in a density gradient of Percoll. Five fractions were isolated, consisting of about 6, 19, 39, 95 and 83% gonadotrops, respectively. The gonadotrops were identified by their ultrastructural characteristics, by immunocytochemistry, and by measuring their hormone content. After one day in culture, in each fraction the secretion of gonadotropin could be stimulated by a luteinizing hormone-releasing hormone analogue, indicating that the cells had retained their functional integrity. Since the regulatory mechanisms of different cell types from the pituitary have some similarity, purification of the gonadotrops provides a model to study the regulation of gonadotropin secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217237
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The brain-pituitary-gonadal axis in the rainbow trout, Salmo gairdneri (1983)
    Springer
    Cell & tissue research 233 (1983), S. 377-388 
    Details
    ISSN: 1432-0878
    Keywords: Gonadotrops ; Gonadotropin secretion ; Steroids ; Brain-pituitary-gonadal axis ; Juvenile teleost
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In a cytophysiological study it was investigated whether in juvenile trout gonadal steroids stimulate the gonadotropic (GTH)-cells directly or indirectly via the brain. Pituitaries of donor animals were transplanted into the caudal musculature of testosterone-treated and non-testosterone-treated host fish. Testosterone treatment caused an increase in GTH-content in the in situ pituitaries and in the grafts. Accordingly, the gonadotrops displayed ultrastructural changes such as the appearance of well-developed Golgi systems and large globules. The stimulation of the morphological development of gonadotrops and of synthesis and storage of GTH in the allografted pituitaries indicates that testosterone affects the GTH-cells directly. In untreated juvenile trout the gonadotropin content of the pituitary and the gonadotropin concentration in the plasma vary with the time of year. This variation and the role of testosterone and gonadotropin-releasing hormone on the release of GTH are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00238304
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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