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  • 1
    Electronic Resource
    Electronic Resource
    Somatic hybridization of Nicotiana tabacum and Petunia hybrida (1986)
    Springer
    Molecular genetics and genomics 202 (1986), S. 342-347 
    Details
    ISSN: 1617-4623
    Keywords: Protoplasts ; Fusion ; Hybrids ; Nicotiana ; Petunia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Leaf mesophyll protoplasts of a nitrate reductase deficient streptomycin resistant mutant of Nicotiana tabacum were fused with cell suspension protoplasts of wild type Petunia hybrida. Somatic hybrid cell colonies were selected for streptomycin resistance and nitrate reductase proficiency. Six independent cell lines, capable of growth in selection medium, were analysed by electrophoresis of callus peroxidases and leucine aminopeptidases and also by hybridization with rDNA and a chloroplast encoded gene as molecular probes. The results show that all six lines represented nuclear somatic hybrids, possessing the chloroplast of N. tabacum, at an early stage of development. However, after 6–12 months in culture, genomic incompatibility was observed resulting in the loss of most of the tobacco nuclear genome in the majority of the cell lines. One of the latter cell lines regenerated plants which possessed the chloroplast of N. tabacum in a predominantly P. hybrida nuclear background.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333260
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Assessment of the efficiency of cotransformation of the T-DNA of disarmed binary vectors derived from Agrobacterium tumefaciens and the T-DNA of A. rhizogenes (1987)
    Springer
    Plant molecular biology 9 (1987), S. 573-584 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium rhizogenes ; binary vector ; kanamycin resistance ; transformation ; secondary metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Co-transfer of Agrobacterium rhizogenes T-DNA and T-DNA from the A. tumefaciens binary vector pBin19 (Bevan, 1984) was studied in detail using Nicotiana rustica. High frequencies of co-transfer of T-DNA's were observed, even when no selection pressure was exerted. Increased levels of pBin19 T-DNA were found in hairy root cultures with selection at higher levels of kanamycin sulphate (50–200 μg ml−1). Several other species were also transformed by A. rhizogenes carrying pBin19 and A. rhizogenes harbouring a different binary factor, pAGS125 (Van den Elzen et al., 1985), was used to transform N. rustica hairy roots to confer hygromycin B resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020534
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cryopreservation and post freeze molecular and biosynthetic stability in transformed roots of Beta vulgaris and Nicotiana rustica (1991)
    Springer
    Plant cell, tissue and organ culture 24 (1991), S. 163-172 
    Details
    ISSN: 1573-5044
    Keywords: Agrobacterium rhizogenes ; cryopreservation ; hairy roots ; molecular stability ; secondary metabolites ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Crypopreservation methods were firstly developed for root-tips from hairy root cultures of Beta vulgaris, established after transformation by Agrobacterium rhizogenes. The effects of culture age, pre-growth, cryoprotection, freezing rate and post-freeze culture conditions were determined. The resulting freezing protocol was then used to cryopreserve transformed root cultures of Nicotiana rustica. Both species were viable after freezing (ca. 80%), according to fluorescein diacetate vital staining. However, on average the regeneration of proliferating roots from surviving root-tips was low (〈20%). Growth rates, secondary metabolite production and T-DNA structure of a number of hairy root lines were examined and found to be unchanged after cryopreservation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033472
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    Paper (German National Licenses)
    Fulltext
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