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  • 1
    Electronic Resource
    Electronic Resource
    Thionins: properties, possible biological roles and mechanisms of action (1994)
    Springer
    Plant molecular biology 26 (1994), S. 25-37 
    Details
    ISSN: 1573-5028
    Keywords: Antimicrobial ; ion channel ; phosphoinositides ; plant defence ; thionins ; toxins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thionins are low-molecular-weight proteins (M r ca. 5000) occurring in seeds, stems, roots and leaves of a number of plant species. The different members of this family of plant proteins show both sequence and structural homology, and are toxic to bacteria, fungi, yeasts and various naked cells in vitro. Toxicity requires an electrostatic interaction of the positively charged thionin with the negatively charged phospholipids making up the membrane, followed by either pore formation or a specific interaction with a certain lipid domain. This domain might be composed of phosphoinositides, which mediate transduction of environmental signals in eukaryotes. Their in vitro toxicity to plant pathogenic bacteria and fungi could reflect a direct role in plant defence, although, in view of the many divergent activities displayed by thionins both in vitro and in vivo, a biological role other than inhibition of microbial growth is equally plausible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039517
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Fate of introduced genetic markers in transformed root clones and regenerated plants of monohaploid and diploid potato genotypes (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 185-193 
    Details
    ISSN: 1432-2242
    Keywords: Potato ; Agrobacterium transformation ; Plant regeneration ; Monohaploid, diploid ; Genetic markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Agrobacterium transformation of stem internodes of four monohaploid (839-79, 849-7, 851-23, 855-1) and two diploid (M9 and HH260) potato genotypes using hairy root-inducing single (LBA 1020, LBA 9365, LBA 9402) and binary (LBA 1060KG) vectors is reported. Various media and successive culture steps were tested for plant regeneration from different transformed root clones. The fate of introduced genetic markers in root clones and regenerated plants (hairy root phenotype, hormone autotrophy, opine production, kanamycin resistance, β-glucuronidase activity), the ploidy stability and protoplast yield were analysed. The transformation efficiency of stem internodes (hairy root production) and the regeneration capacity of the transformed root clones greatly differed within and between the various potato genotypes. The regenerated plants obtained after transformation with both types of vectors often showed the absence of one or more genetic markers. However, transformation with the binary Agrobacterium vector generally resulted in the stable presence of the opines in all transformed root clones and most regenerated plants. In HH260, transformation efficiency, plant regeneration of transformed root clones, protoplast yield and ploidy stability were the highest as compared to the other genotypes. The application of these transformed plants as marker lines in gene mapping and gene expression studies is indicated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288798
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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