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  • 1
    Electronic Resource
    Electronic Resource
    Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious shoot regeneration on leaf and stem segments (1989)
    Springer
    Plant molecular biology 12 (1989), S. 329-337 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium tumefaciens ; adventitious shoot regeneration ; transformation ; homozygous potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transformed potato (Solanum tuberosum) plants were obtained from homozygous diploid potato by using a transformation procedure in combination with an adventitious shoot regeneration method. Leaf and stem explants were inoculated with an Agrobacterium tumefaciens strain which contained a binary vector (pVU 1011) carrying the neomycin phosphotransferase gene. Shoot regeneration most effectively on stem explants, occurred within six weeks directly from the explants without introducing a callus phase. A strong seasonal influence on transformation efficiencies was observed. Analysis of a number of randomly selected regenerated shoots for their ability to root and form shoots on kanamycin-containing medium shows that over 90% of the regenerated shoots obtained are transformed. In a number of shoots transformation was confirmed by a test for the presence and expression of the NPT-II gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043210
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Field evaluation of antisense RNA mediated inhibition of GBSS gene expression in potato (1991)
    Springer
    Euphytica 59 (1991), S. 83-91 
    Details
    ISSN: 1573-5060
    Keywords: Agrobacterium rhizogenes ; antisense RNA ; granule-bound starch synthase ; Solanum tuberosum ; starch composition ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Granule-bound starch synthase (GBSS) catalyses the synthesis of amylose in starch granules. Analysis of antisense RNA mediated inhibition of GBSS gene expression in large numbers of tubers from in vitro grown, greenhouse grown and field grown transgenic potato plants revealed stable and total inhibition of GBSS gene expression in one clone. In three other transgenic genotypes partial and unstable inhibition was found. In these genotypes both GBSS activity and amylose content were remarkably reduced compared with the non-transformed control genotype. No relationship was found between the level of inhibition of GBSS gene expression and yield and dry matter content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025364
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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