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  • hybrids  (2)
  • Agropyron elongatum  (1)
  • Puccinia recondita f.sp. tritici  (1)
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  • 1
    ISSN: 1432-2242
    Keywords: Leaf rust ; RFLP ; RAPD ; Wheat ; Agropyron elongatum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The objective of this study was to identify molecular markers linked to the wheat leaf rust resistance gene Lr24 derived from Agropyron elongatum (3DL/3Ag translocation). Two near isogenic lines (NILs), ‘Arina’ and Lr24/7 * “Arina”, were screened for polymorphism at the DNA level with 115 RFLP probes. Twenty-one of these probes map to the homoeologous group 3. In addition, 360 RAPD primers were tested on the NILs. Six RFLP probes showed polymorphism between the NILs, and 11 RAPD primers detected one additional band in the resistant NIL. The genetic linkage of the polymorphic markers with Lr24 was tested on a segregating F2 population (150 plants) derived from a cross between the leaf rust resistant Lr24/7 * “Arina” and the susceptible spelt (Triticum spelta) variety ‘Oberkulmer’. All 6 RFLP markers were completely linked to Lr24: one was inherited as a codominant marker (PSR1205), one was in coupling phase (PSR1203) and 4 were in repulsion phase (PSR388, PSR904, PSR931, PSR1067) with Lr24. The localization of these probes on chromosome 3D was confirmed by nulli-tetrasomic analysis. Distorted genotypic segregation was found for the Codominant RFLP marker PSR1205. This distortion can be explained by the occurrence of hemizygous plants. One of the 11 RAPD markers (OPJ-09) also showed complete linkage to theLr24 resistance gene. The polymorphic RAPD fragment was cloned and sequenced. Specific primers were synthesized, and they produced an amplification product only in the resistant plants. This specific marker allows a reliable and rapid screening of a large number of genotypes in practical breeding. Analysis of 6 additional lines containing Lr24 revealed that 3 lines have a smaller chromosomal segment of A. elongatum than lines derived from ‘Agent’, a commonly used gene donor for the Lr24 resistance gene.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 45 (1990), S. 87-92 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; near-isogenic lines ; NILs ; Puccinia recondita f.sp. tritici ; leaf rust ; Puccinia striiformis ; yellow rust ; backcross ; variation ; background resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Using the cultivar Arina as the recurrent parent, six backcrosses were made with two donor lines carrying the leaf rust resistance genes Lr1 and Lr9, respectively. Selection for leaf rust resistance occurred at the seedling stage in the greenhouse; the first plants transferred to the field were BC6F4s. Frequency distribution of the 332 Lr1/7 × Arina and the 335 Lr9/7 × Arina lines showed continuous variation for yellow rust resistance and heading date in these leaf rust near-isogenic lines (NILs). Similar results were also obtained for plant height, for resistance to powdery mildew and glume blotch, as well as for baking quality characters in another set of more advanced NILs. The available information on the behaviour of one of the parents of cultivar Arina led to the conclusion that the expressed yellow rust resistance is quantitative and might possibly be durable.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5060
    Keywords: wheat ; spelt ; crosses ; hybrids ; quality ; disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary With the objective of creating new combinations of disease resistance and quality, hybrids between wheat and spelt (spelt is well adapted to cool and wet conditions) were produced and the expression of heterosis was analysed. Three winter wheat varieties were crossed reciprocally with two spelt cultivars and the F1 hybrids were tested under artificial inoculation with stripe rust, powdery mildew and leaf rust. Disease susceptibility and quality characters (protein content, Zeleny value, grain hardness) were assessed in two year field trials. For stripe rust the F1 hybrids were resistant if one of the wheat parents was resistant. Combinations with the susceptible wheat cultivar ‘Arina’ were all susceptible irrespective of using a resistant spelt partner. Although the infection with powdery mildew was rather low, a similar reaction was obtained with the susceptible wheat variety ‘Bernina’. Leaf rust revealed very specific varietal influences. The two susceptible wheat varieties ‘Bernina’ and ‘Arina’ resulted in susceptible F1 hybrids when combined with a moderately resistant spelt. Only when they were crossed with a resistant spelt cultivar the F1 hybrids were resistant. ‘Forno’, a leaf rust resistant wheat, gave resistant F1 hybrids in all combinations. Without exception the quality characters tested showed a negative heterosis effect resulting in protein levels and Zeleny values close to or below the values of the lower parent. It appears to be possible to produce resistant F1 hybrids, mostly dominated by the resistance level of the wheat partner. The quality of the hybrids is mainly suitable for biscuit and spelt specific products; it needs specific screening for combinations with acceptable breadmaking quality.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5060
    Keywords: hybrids ; crosses ; spelt ; Triticum spelta ; wheat ; Triticum aestivum ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary F1 and F2 hybrids were produced using three winter wheat varieties (Bernina = biscuit wheat, Arina and Forno = bread wheat) and two spelt cultivars (Oberkulmer and Rouquin). Data are based on field trials in 1989 and 1990. All the combinations tested gave a very high relative heterosis on grain yield per ear, varying between 29.9% and 47.6% with an average of 42.1%. Even the higher parent was outyielded by 24.1% to 45.7%. This was the result of a heterosis effect on the two yield parameters grain number per ear and single grain weight. In the F2, heterosis for grain yield per ear was not significant with a range between 3.9% and 12.0%. F1 hybrids showed a heterosis effect on plant height but no hybrid was taller than the spelt parents. These data show that the gene pool of spelt could be potentially usefull for the selection of parents for hybrid production. For practical applications there remain several problems to be solved. Despite the very high heterosis effect the yield potential of spelt needs to be improved. No short spelt varieties exist at present to produce hybrids short enough for commercial application. Technical problems in the seed production arise because of the close tightness of the spelt glumes hampering either the pollination by the wheat parent or the pollen dispersal if used as a pollinator. However, a breeding effort to select for improved parents within the gene pool of spelt appears useful for future F1 hybrids between spelt and wheat.
    Type of Medium: Electronic Resource
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