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  • Aircraft measurements  (2)
  • Legionella pneumophila  (2)
  • Enzyme kinetics  (1)
  • 1
    ISSN: 0014-5793
    Keywords: Enzyme kinetics ; FK506-binding protein ; Legionella pneumophila ; Mip protein ; Oligomerization ; Peptidyl-prolyl cis/trans isomerase ; Virulence factor
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-1472
    Keywords: Air pollution meteorology ; Aircraft measurements ; Field experiment ; Lidar measurements ; Plume diffusion ; Sea breeze ; Thermal internal boundary layer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Fumigation under sea-breeze conditions is a major feature of the air pollution meteorology in the coastal industrial region of Kwinana, south of Perth in Western Australia. An intensive field experiment on fumigation was carried out in the region in early 1995 with the objective of using the measured data to develop and test a shoreline fumigation model. Fumigation of plumes from the Kwinana Power Station was studied using an instrumented research aircraft, radiosonde balloons, meteorological stations, a lidar, a mobile surface sampler, and sonic anemometers. The study has yielded a detailed and high quality data set as a result of both the range of observations undertaken and of the regularity of the sea-breeze conditions under scrutiny. The details of the experiment are summarised in this paper and some typical results are presented.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1617-4623
    Keywords: Key words Green fluorescent protein (GFP) ; Legionella pneumophila ; Intracellular survival ; Macrophage infectivity potentiator (Mip) protein ; Acanthamoeba castellanii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The gene encoding the green fluorescent protein (GFP) was used as a reporter gene in Legionella pneumophila. To analyze GFP expression in Legionella, transcriptional fusions of gfp with the Legionella-specific mip (macrophage infectivity potentiator) promoter (P mip ) and the sod (superoxide dismutase) promoter (P sod ) derived from Listeria monocytogenes were constructed. Following transformation into the virulent L. pneumophila strain JR 32, strong GFP-mediated fluorescence was detected with both plasmids, although the sod promoter was asssociated with a 1ten-fold higher intensity. No fluorescence was observed in L. pneumophila transformed with the promoterless gfp gene. Comparison of fluorescence yields between various L. pneumophila strains that differ in their virulence characteristics and were transformed with the P mip -gfp carrying plasmid revealed no differences in GFP expression. Infection studies using Acanthamoeba castellanii as host and recombinant L. pneumophila strains carrying the P mip -gfp and P sod -gfp fusions indicated that the mip promoter was expressed when the bacteria replicated intracellularly. GFP expression was also used to monitor, in infected A. castellanii cells, the intracellular survival of, and incidence of host-cell killing by, L. pneumophila strains that vary in their virulence properties. As quantified by flow cytometry the highly virulent L. pneumophila strain Corby was twice as infectious to A. castellanii as the Philadelphia strain JR 32. Using the avirulent Philadelphia derivative 25D invasion but no intracellular multiplication was observed. In addition, we examined by flow cytometry the influence of cytochalasin D, cycloheximide, and methylamine on the uptake of Legionella by A. castellanii. In conclusion, gfp appears to be a convenient reporter gene whose expression in Legionella can be followed in real time and allows analysis of promoter activities in Legionella and monitoring of the infection process.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Boundary layer meteorology 89 (1998), S. 385-405 
    ISSN: 1573-1472
    Keywords: Aircraft measurements ; Atmospheric dispersion ; Coastal meteorology ; Sea breeze ; Shoreline fumigation ; Slab model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Aircraft measurements of potential temperature and turbulent kinetic energy are used to examine the growth of the thermal internal boundary layer (TIBL) in sea-breeze flows on four selected days of a coastal fumigation study performed in 1995 at Kwinana in Western Australia. The aircraft data, together with radiosonde measurements taken on the same days, show a multi-layered low-level onshore flow in the vertical with a superadiabatic layer extending to about 50 m above the water surface on all four days. On the first three days the layer above the superadiabatic layer was neutral, typically 200 m deep, capped by a stably stratified region, whereas on the remaining day it was fully stable. The occurrence of the neutral layer on most experimental days contrasts with the more usual situation involving an entirely stable onshore flow. A composite approach based on both temperature and turbulence data is used to provide a pragmatic but self-consistent definition of the TIBL height. The data for the first three days indicate that the TIBL grows rapidly into the neutrally stratified region to the top of the region within about 2 km from the coast, with a very slow subsequent growth into the stable stratification aloft. On the other hand, the TIBL grows only to about 200 m within a distance of 7 km from the coast on the fourth day due to a strong stable stratification. An existing numerical TIBL model based on the slab approach, capable of describing the TIBL growth in both neutral and stable environments, and a recent analytical model, more efficient for operational use, are used to simulate the aircraft TIBL observations. The predictions by both models agree reasonably well with the data.
    Type of Medium: Electronic Resource
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