ISSN:
1432-072X
Keywords:
Desulfovibrio
;
Alanine degradation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Desulfovibrio HL21 is unable to grow with amino acids as energy substrates. Alanine, serine, aspartate and to some extent glutamate were used as carbon and nitrogen sources in the presence of hydrogen as the energy substrate. Dense cell suspensions converted alanine stoichiometrically to acetate, NH 4 + and presumably HCO 3 - , but at a very low rate. Desulfovibrio HL21 cells grown with alanine as carbon and nitrogen source contained increased levels of NAD(P)-dependent l-alanine dehydrogenase as compared to cells grown with NH4Cl as nitrogen source. Unfavourable kinetic properties of this alanine dehydrogenase, repression of the synthesis of the enzyme by NH 4 + and a low rate of NADH oxidation all have a negative effect on the rate of degradation of alanine and may partly explain the inability of the strain to grow with alanine as an energy substrate.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00443657
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