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  • Biochemistry and Biotechnology  (3)
  • Alfalfa  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Characterization of a new Agrobacterium tumefaciens strain from alfalfa (Medicago sativa L.) (1998)
    Springer
    Archives of microbiology 169 (1998), S. 381-386 
    Details
    ISSN: 1432-072X
    Keywords: Key words Agrobacterium tumefaciens ; Alfalfa ; Medicago sativa ; Ti plasmid ; Virulence ; Octopine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Agrobacterium tumefaciens strain 1D1609 is reported here as the first field isolate from alfalfa (Medicago sativa L.). Unlike well-characterized A. tumefaciens strains such as C58 and Ach5, strain 1D1609 is highly virulent on alfalfa and has a distinctive host range. Interestingly, strain 1D1609 is naturally resistant to kanamycin and spectinomycin. The Ti plasmid in strain 1D1609 is an octopine-type; thus, tumors formed by strain 1D1609 synthesize octopine, which is utilized by the bacterium as a sole carbon source. Reciprocal exchange of Ti plasmids between strains 1D1609 and C58 showed that both chromosomal and Ti plasmid genes in strain 1D1609 contribute specifically to tumor formation on alfalfa. In addition, the nondormant CUF101 alfalfa cultivar from which strain 1D1609 was isolated was significantly more susceptible to all Agrobacterium strains tested than was the dormant Agate cultivar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050586
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A rapid screening technique for identifying murine monoclonal antibodies to human apolipoprotein A-I isoforms by Western blot analysis (1987)
    Weinheim : Wiley-Blackwell
    Electrophoresis 8 (1987), S. 552-556 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A rapid method for determining the reactivity of murine monoclonal antibodies with apolipoprotein A-I (Apo A-I) isoforms has been developed. The method consists of a rapid (1-2 h) prescreening of fusion well culture fluids by using an automated immunofluorescent assay (IF A) to identify fusion well cultures producing monoclonal antibody against Apo A-I and high-density lipoprotein (HDL). Immediately following the IFA procedure, immunodetection of the reactivity pattern of selected fusion well culture fluids is determined by using transblotted isoforms of Apo A-I previously separated by isoelectric focusing. Strips are prepared and stored at 4 °C in protein containing blocking solution before use. Preselected culture fluids could be tested within 6 h for qualitative reactivity with Apo A-I isoforms. Using this procedure we selected several monoclonal antibody-producing cultures for further studies on HDL metabolism based on their differing reactivities with Apo A-I isoforms. This technique should be easily transferable to other laboratories for assessing proteins having multiple isoforms since the crucial steps of separation and blotting can be optimized and performed before actual testing for antibody reactivity.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150081203
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Measurement of dissolved oxygen in fermentationsPublished with the approval of the Director of the Wisconsin Agricultural Experiment Station. Supported in part by funds from Abbott Laboratories, North Chicago, Illinois. (1961)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 261-275 
    Details
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Two methods have been investigated for the measurement of dissolved oxygen in fermentations: the tubing method and the probe method. The sensing elements for both methods are steam-sterilizable and measure only the oxygen tension in the liquid phase. The tubing method is probably the most accurate and reliable method available for measurement of dissolved oxygen in fermentations but it requires extensive ancillary apparatus.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbmte.390030306
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Aeration in fermentations (1961)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 277-309 
    Details
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Symptoms of oxygen deficiency and variations in dissolved-oxygen level have been described in some typical aerobic fermentations. In bacterial fermentations, dissolved-oxygen levels were almost the same in different parts of the fermentor. As a result, good correlations between apparent and true critical oxygen concentrations were obtained. Poor correlations were obtained in mould fermentations.
    Additional Material: 27 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbmte.390030307
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    Paper (German National Licenses)
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