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  • 1
    ISSN: 1573-1561
    Keywords: Allelochemicals ; cabbage looper ; Glycine max ; Lepidoptera ; Noctuidae ; plant resistance ; plant volatiles ; soybeans ; steam distillates ; Trichoplusia ni
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Soybean plant volatiles, extracted as steam distillates, significantly affected the behavior and biology of the cabbage looper,Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae). Distillates from the susceptible Davis variety attractedT. ni larvae and female adults, whereas those from resistant PI 227687 plants repelled them. When mixed in an artificial diet, steam distillates from PI 227687 plants caused mortality of first-instar larvae. Adults emerging from pupae topically treated with 5 μg of such PI 227687 extractable showed developmental abnormalities. Larval feeding was significantly less on Davis leaves treated with PI 227687 volatiles as compared to solvent (acetone) or such Davis extractables. However, Davis volatiles on PI 227687 leaves did not increase larval feeding. HPLC analyses of steam distillates from susceptible Davis versus resistant PI 227687 indicated differences.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-1561
    Keywords: Allelochemicals ; isofiavonoids ; coumestrol ; antixenosis ; Epilachna varivestis ; Mexican bean beetle ; Coleoptera ; Coccinellidae ; Glycine max ; Phaseolus lunatus ; feeding bioassay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The antixenosic properties of the isoflavonoid, coumestrol, were tested in dual-choice leaf disk bioassays with the Mexican bean beetle (Epilachna varivestis Mulsant).E. varivestis preferred the methanol-r,reated (solvent-control) disk when the coumestrol concentration was 1.8 or 0.9μ/leaf disk. No preference was observed between the coumestrol-treated and the solvent-control disks when the coumestrol concentration was higher, at 3.6, or lower, at 0.45μg/leaf disk. Coumestrol alone clearly is not responsible for the significant constitutive antixenosic properties of “Davis” soybeans,Glycine max (L.) Merrill, because the amount of coumestrol in these plants is significantly less than the minimum concentration which was antixenosic in this study. However, it might contribute to a constitutive antixenosis in “Davis” involving a profile of allelochemicals. A computer-aided densitometer, adapted to measure the leaf disk area, increased the resolution of the leaf area 250 (X)-fold as compared to the standard LI-COR leaf area meter.
    Type of Medium: Electronic Resource
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