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  • C-reactive protein  (2)
  • Allergic symptoms  (1)
  • ELISA  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of the allergenic proteins in the pollen of Cryptomeria japonica (1994)
    Springer
    Sexual plant reproduction 7 (1994), S. 95-100 
    Details
    ISSN: 1432-2145
    Keywords: Allergic symptoms ; Allergic Protein ; Cryptomeria japonica ; Cry j I ; Immunocytochemystry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Applying an immunocytochemical method, a localization of the protein Cry j I in the Cryptomeria japonica pollen, which is the major allergen responsible for Japanese cedar pollinosis, is investigated with the monoclonal and polyclonal antibodies produced from the protein. The protein that reacts to the polyclonal antibody localizes on the sexine, nexine, between nexine and intine layers, orbicles, cell wall of a generative cell, Golgi body and Golgi vesicles. The allergenic protein contained in the exine and orbicles of Japanese cedar pollen can diffuse or dissolve easily from there into the mucus covering of the eye and nose, causing a response in less than 1 min after exposure. Since the orbicles have a diameter of about 430 nm, they can pass easily through the pores of most protective masks to reach the sensitive tissues of the patient. The proteins react to the monoclonal antibodies (J1BO1 and J1BO7) and localize on the Golgi body, sexine, nexine and orbicles (but not between the nexine and intine layers), and on the generative cell wall. In the young pollen grain, numerous allergenic protein particles contained in the orbicles and sexine layer, but there is only a small amount of the protein between the nexine and intine layers, since the intine layer is not yet complete at this stage. More will be accumulated there during developmental maturation. The allergenic protein is also found on the tapetal materials remaining in the young anther. Since the materials forming the exine layer and orbicles come from tapetal tissue, it is assumed that some of the allergenic protein is produced in the tapetum and localized in the orbicles and pollen wall during maturation, and that the rest of the allergenic protein is produced in the Golgi body in the mature pollen grain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00230577
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Immunological determination of faecal haemoglobin concentrations in dogs (1998)
    Springer
    Veterinary research communications 22 (1998), S. 193-201 
    Details
    ISSN: 1573-7446
    Keywords: diagnosis ; dog ; ELISA ; faeces ; latex agglutination ; occult blood ; serology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Faecal haemoglobin (Hb) concentrations in apparently healthy experimental Beagle dogs and in dogs of various breeds kept in private households or at breeders were measured by reversed passive latex agglutination (RPLA) and enzyme-linked immunosorbent assay (ELISA) in an effort to define the physiological concentrations of faecal Hb in the dog. In 88% (53) of 60 experimental Beagle dogs (30 males and 30 females), the RPLA titres were 1:2 and 1:8 and the faecal Hb concentrations ranged from 40.0 to 431.5 (mean 184.1±92.6) μg/g faeces by ELISA. No significant difference was found in Hb levels or RPLA titres between males and females. Seven dogs (12%) had significantly greater RPLA titres and Hb concentrations by ELISA than the remaining dogs. In 84% (45) of the 53 dogs kept in private households or at breeders, the RPLA titres were 〉1:1 to 1:8 and the faecal Hb concentrations ranged from 7.1 to 456.7 (mean 137.5±128.7) μg/g faeces in ELISA. Eight of these dogs (15.1% of 53 dogs) had significantly greater RPLA titres and Hb concentrations by ELISA than the remaining dogs. There were no significant differences between the Beagles and dogs kept in private households or at breeders. In conclusion, in 98 (86.7% of 113) dogs the physiological concentrations of RPLA titres were 〉1:1 to 1:8 and the faecal Hb concentrations were 143.5–185.1 μg/g (95% confidence level). Approximately 13.3% of apparently healthy dogs had higher faecal Hb concentrations, suggesting the presence of subclinical haemorrhages. Four dogs suffering from colorectal cancer also had high faecal Hb concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006025422317
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Changes in serum C-reactive protein levels in dogs with various disorders and surgical traumas (1993)
    Springer
    Veterinary research communications 17 (1993), S. 85-93 
    Details
    ISSN: 1573-7446
    Keywords: acute phase proteins ; dogs ; C-reactive protein ; enzyme-linked immunoabsorbent assay ; inflammation ; serum ; surgery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The serum levels of C-reactive protein (CRP) produced as an inflammatory response in dogs with various disorders and surgical traumas were measured by enzyme-linked immunoabsorbent assay and slide reversed passive latex agglutination test (RPLA). The CRP levels were greatly increased 1–2 days after surgery in most of the dogs (n=29) subjected to surgery. These levels had markedly decreased by the time the sutures were removed. In dogs with various disorders (n=58), the serum CRP levels at first diagnosis were high in infectious diseases. In dogs from which paired serum samples were examined, the serum CRP usually showed a decrease with improvement in the condition (n=11) or a terminal increase (n=4) but, conversely, some showed an increase with improvement in the condition (n=3).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01839236
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Serum C-reactive protein and immune responses in dogs inoculated withBordetella bronchiseptica (phase I cells) (1994)
    Springer
    Veterinary research communications 18 (1994), S. 347-357 
    Details
    ISSN: 1573-7446
    Keywords: antibody ; Bordetella bronchiseptica ; bronchopneumonia ; C-reactive protein ; dogs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Eight Beagle dogs were inoculated intrabronchially with 5×109 live, avirulent cells ofBordetella bronchiseptica L-414 strain (phase I cells) (B. bronchiseptica) to investigate the serum levels of their C-reactive protein, the white blood cell counts, the antibody responses toB. bronchiseptica in the sera and tracheal secretions, and the effects of prednisolone given to four of the dogs on C-reactive protein (CRP), white blood cells (WBC) and immune responses. In two Beagle dogs inoculated intrabronchially with sterile physiological saline, the concentrations of CRP and the WBC counts did not increase. CRP was markedly increased one day after inoculation in the dogs inoculated withB. bronchiseptica to 385.0–720.0 µg/ml (mean 498±132 µg/ml) in the group given theB. bronchiseptica inoculation only, and to 372.0–649.0 µg/ml (mean 551±106 µg/ml) in the group treated with prednisolone following inoculation ofB. bronchiseptica, as determined by an enzyme-linked immunosorbent assay (ELISA). The CRP levels were 23–95 times the pre-inoculation values, which indicated that prednisolone had no effect on the production of CRP. In the prednisolone-treated group, the WBC count increased and stayed at an increased level for approximately 12 days. An indirect fluorescent antibody test led to the detection of anti-B. bronchiseptica IgM and IgG antibodies in the sera from 5 days afterB. bronchiseptica inoculation and S-IgA and IgG anti-B. bronchiseptica antibodies in the tracheal secretions on the day after the challenge exposure toB. bronchiseptica. The increase in CRP after challenge exposure toB. bronchiseptica was significantly (p〈0.05) smaller than that found after the first inoculation ofB. bronchiseptica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01839285
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    Paper (German National Licenses)
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