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  • 1
    Electronic Resource
    Electronic Resource
    An Arabidopsis homologue of the Drosophila meiotic gene Pelota (2000)
    Springer
    Sexual plant reproduction 12 (2000), S. 310-313 
    Details
    ISSN: 1432-2145
    Keywords: Key words Meiosis ; Arabidopsis ; Pelota
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have identified a plant homologue of the Drosophila meiotic gene Pelota in Arabidopsis thaliana (AtPelota1). This gene maps to chromosome 4 of Ara- bidopsis and is one of two Pelota homologues present in this plant. When the expression pattern of AtPelota1 was examined it was found to be expressed at similar levels in all plant tissues tested (whole plant, bud, stem, leaf and root). This expression pattern corresponds to that seen for some other Arabidopsis meiotic genes and their homologues. A search of the databases reveal that the AtPelota gene family is widespread with homologues present in higher and lower eukaryotes and archaebacteria, but not eubacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050200
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Comparative Analysis of Crossover Exchanges and Chiasmata in Allium Cepa × Fistulosum After Genomic In Situ Hybridization (GISH) (1998)
    Springer
    Chromosome research 6 (1998), S. 567-574 
    Details
    ISSN: 1573-6849
    Keywords: Allium ; chiasmata ; crossing over ; genomic in situ hybridization ; recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genomic in situ hybridization (GISH) successfully differentiated homoeologous genomes in the interspecific hybrid Allium cepa × fistulosum, thus allowing the detection of reciprocal crossover events as label exchanges in separating anaphase I chromosomes. Three of the eight chromosome pairs were positively identified by fluorescence in situ hybridization (FISH) to rDNA sequences. There was a general similarity of the GISH-based label exchange frequencies and metaphase I chiasma frequencies, but with a 20% deficit of chiasmata. Reasons for this apparent deficit are discussed. The locations of chiasmata and label exchanges are in broad agreement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009296826942
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cytological characterization of four meiotic mutants of Arabidopsis isolated from T-DNA-transformed lines (1997)
    Springer
    Chromosome research 5 (1997), S. 551-559 
    Details
    ISSN: 1573-6849
    Keywords: Arabidopsis ; fertility ; meiosis ; mutants ; T-DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A secondary screen of the Feldmann collection of T-DNA transformed Arabidopsis lines identified several meiotic mutants. We used a spreading technique combined with DAPI staining in a detailed cytogenetic analysis of meiotic chromosome behaviour in four of these mutants, all of which are putatively T-DNA tagged and therefore candidates for molecular and functional analysis of the mutated genes. Two of them are defined as ‘synaptic’ mutants, showing greatly reduced association of homologous chromosomes at metaphase I: one is asynaptic, showing failure of synapsis during prophase I, whereas the other is desynaptic and is characterized by normal but non-maintained synapsis. Another mutant is defective in meiotic cell cycle control and undergoes a third meiotic division, resembling a second division but without an additional round of chromosome duplication. A further mutant shows meiosis-limited chromosome disruption, resulting in extensive chromosome fragmentation combined with other defects. All four mutants experience very irregular chromosome distribution during the meiotic divisions, resulting in abnormal numbers and/or sizes of microspores, with resulting reduced fertility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018497804129
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  • 4
    Electronic Resource
    Electronic Resource
    Distribution of a 375 bp repeat sequence inAllium (Alliaceae) as revealed by FISH (1999)
    Springer
    Plant systematics and evolution 217 (1999), S. 31-42 
    Details
    ISSN: 1615-6110
    Keywords: Alliaceae ; Allium ; Fluorescent in-situ hybridisation ; cytotaxonomy ; telomeric repeat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Information about evolutionary relationships between species of the genusAllium is desirable in order to facilitate breeding programmes. One approach is to study the distribution of repetitive DNA sequences among species thought on taxonomic grounds, to be closely related. We have used fluorescent in-situ hybridisation (FISH) to examine seven species within sect.Cepa of the genus (A. altaicum, A. cepa, A. fistulosum, A. galanthum, A. pskemense, A. oschaninii andA. vavilovii), one species from sect.Rhizirideum (A. roylei), two species from sect.Allium (A. sativum andA. porrum) and one species from sect.Schoenoprasum (A. schoenoprasum). Each species was probed using a 375 bp repeat sequence isolated fromA. cepa (Barnes & al. 1985), which was generated and labelled by polymerase chain reaction (PCR). No signals were detected in anyAllium species not belonging to sect.Cepa with the exception ofA. roylei, whose designation in sect.Rhizirideum is now questioned. Within sect.Cepa the probe was found to hybridize to the ‘terminal’ regions of the chromosome arms of all the species examined. In addition a number of interstitial bands were detected. Use of FISH reveals a more detailed map of the location of the repeat sequences than has previously been obtained by C-banding and other staining procedures. The distribution of the terminal and interstitial sites when compared, allow us to identify three species groups namely,A. altaicum andA. fistulosum; A. cepa, A. roylei, A. oschaninii andA. vavilovii; andA. galanthum andA. pskemense.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984920
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    Paper (German National Licenses)
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