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  • Biodiversity  (3)
  • Allium  (2)
  • CCA  (2)
  • RFLP  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 100 (2000), S. 607-613 
    ISSN: 1432-2242
    Keywords: Key words AFLP ; Biodiversity ; Genetic maps ; Genetic resources ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Genetic diversity within populations of organisms and species is commonly measured using molecular-marker data. It has been claimed that more reliable diversity measurements can be obtained using selected genetically mapped markers to ensure that all regions of the genome are represented in the data sets employed. However, this has not been tested. In the present study, using rice (Oryza sativa L.) as a model species, we have shown that the use of unmapped AFLP markers reveals a pattern of diversity that is very similar to that obtained using a range of other marker types and which reflects the known crossability groups within this species. In contrast, we show that use of mapped-marker data can, in some cases, result in highly misleading patterns of diversity; the results obtained are critically related to the choice ofparents used in the cross from which the mapping population was produced. For diversity analyses, we propose that it is appropriate to use unmapped markers provided that the marker-type has been shown to have a wide distribution over the genome.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Sugar beet ; Beta nana ; Beta vulgaris ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary cDNA probes have been used to assess genetic variation in beet using hybridisation techniques that detect restriction fragment length polymorphism. Probes have been identified which differ in the levels of variation that they can detect (i) within closely related genetic material of sugar beet, and (ii) between sugar beet and a taxonomically distant Beta species.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 84 (1992), S. 113-117 
    ISSN: 1432-2242
    Keywords: Beet ; Somaclonal variation ; Isozyme ; RFLP ; Genetic stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sugar, fodder and garden beet (Beta vulgaris L.) plants have been regenerated in culture from a range of expiant material. Of the regenerants 764 were subjected to isozyme analysis using eight enzyme-specific stains, and 60 were subjected to RFLP analysis using three cDNA probes. Both molecular techniques allowed the identification of somaclonal variant plants. Assessment of the numbers of variant isozymes and restriction fragments has allowed the calculation of the approximate percentage of variant alleles occurring in any one somaclonal regenerant, namely between 0.05% and 0.1%.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2242
    Keywords: Chamaecytisus ; Tagasaste ; Fodder legumes ; Multivariate analysis ; RAPD ; Plant genetic resources ; Biodiversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Random Amplified Polymorphic DNA (RAPD) was used to generate molecular markers to trace the origin of the fodder legume tagasaste (Chamaecytisus proliferus (L. fil.) Link ssp. palmensis (H. Christ) Kunkel) in the Canary Islands. Results from multivariate analyses of data through “Two Way Indicator Species Analysis” (TWINSPAN) and “Detrended Correspondence Analysis” (DECORANA) showed that genotypes collected on the island of La Palma exhibited a wider range of variation than those from the other islands. This supports the existing hypothesis that tagasaste originated on La Palma and emphasizes the importance of conserving and evaluating germ plasm from this island.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5052
    Keywords: Ecogeography ; Fodder-legumes ; In-situ-conservation ; Biodiversity ; Germplasm ; Tagasaste
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chamaecytisus proliferus (L.fil.) Link (Fabaceae: Genisteae) represents a species complex in the Canary Islands. Floristic data from 147 releves from the whole complex were collected and analysed by classification (TWINSPAN) and ordination (DECORANA) methods. Results indicate that white escobon of Tenerife, escobon of El Hierro, white escobon of Gran Canaria and typical tagasaste in La Palma are associated with those plant communities from the north of these islands which are under the influence of the north-eastern trade winds. Narrow-leaved escobon in Tenerife and La Gomera, escobon of southern Gran Canaria and white tagasaste of La Palma are found in those areas which are not under the direct influence of these winds. Morphological forms from the more easterly islands (Gran Canaria and Tenerife-La Gomera) have the broadest ecological range and they have played an important role in the floristic changes which have taken place after the destruction of the forests in these islands. The highest priorities for in situ conservation should be given to wild populations of typical tagasaste, white escobon of Tenerife and escobon of El Hierro.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-6849
    Keywords: Allium ; chiasmata ; crossing over ; genomic in situ hybridization ; recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genomic in situ hybridization (GISH) successfully differentiated homoeologous genomes in the interspecific hybrid Allium cepa × fistulosum, thus allowing the detection of reciprocal crossover events as label exchanges in separating anaphase I chromosomes. Three of the eight chromosome pairs were positively identified by fluorescence in situ hybridization (FISH) to rDNA sequences. There was a general similarity of the GISH-based label exchange frequencies and metaphase I chiasma frequencies, but with a 20% deficit of chiasmata. Reasons for this apparent deficit are discussed. The locations of chiasmata and label exchanges are in broad agreement.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 217 (1999), S. 31-42 
    ISSN: 1615-6110
    Keywords: Alliaceae ; Allium ; Fluorescent in-situ hybridisation ; cytotaxonomy ; telomeric repeat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Information about evolutionary relationships between species of the genusAllium is desirable in order to facilitate breeding programmes. One approach is to study the distribution of repetitive DNA sequences among species thought on taxonomic grounds, to be closely related. We have used fluorescent in-situ hybridisation (FISH) to examine seven species within sect.Cepa of the genus (A. altaicum, A. cepa, A. fistulosum, A. galanthum, A. pskemense, A. oschaninii andA. vavilovii), one species from sect.Rhizirideum (A. roylei), two species from sect.Allium (A. sativum andA. porrum) and one species from sect.Schoenoprasum (A. schoenoprasum). Each species was probed using a 375 bp repeat sequence isolated fromA. cepa (Barnes & al. 1985), which was generated and labelled by polymerase chain reaction (PCR). No signals were detected in anyAllium species not belonging to sect.Cepa with the exception ofA. roylei, whose designation in sect.Rhizirideum is now questioned. Within sect.Cepa the probe was found to hybridize to the ‘terminal’ regions of the chromosome arms of all the species examined. In addition a number of interstitial bands were detected. Use of FISH reveals a more detailed map of the location of the repeat sequences than has previously been obtained by C-banding and other staining procedures. The distribution of the terminal and interstitial sites when compared, allow us to identify three species groups namely,A. altaicum andA. fistulosum; A. cepa, A. roylei, A. oschaninii andA. vavilovii; andA. galanthum andA. pskemense.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 400 (1999), S. 195-203 
    ISSN: 1573-5117
    Keywords: macroinvertebrate ; Caribbean ; CCA ; multivariate ; stream
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The macroinvertebrate fauna from 13 river catchments in St. Lucia were sampled over a two year period. Multivariate techniques were used to detect the influence of farming activities and urban developments on macroinvertebrate community variation. Land use was the most significant correlate influencing the pattern of macroinvertebrate community variation. Stream chemistry was insignificant, with the exception of turbidity. Agricultural activity in the upper and middle catchments caused middle and lower stations to suffer more from the severity of spates and these had a deleterious affect on stream communities. Pollution sensitive fauna were more closely associated with undisturbed stations. Results from a hybrid analysis suggested that a significant variable was not included in the analysis. It is likely that hydrobiological data would have been a significant correlate in explaining macroinvertebrate community variation, as the spate frequency regime has been identified as a significant correlate in other sub/tropical environments.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 400 (1999), S. 195-203 
    ISSN: 1573-5117
    Keywords: macroinvertebrate ; Caribbean ; CCA ; multivariate ; stream
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The macroinvertebrate fauna from 13 river catchments in St. Lucia were sampled over a two year period. Multivariate techniques were used to detect the influence of farming activities and urban developments on macroinvertebrate community variation. Land use was the most significant correlate influencing the pattern of macroinvertebrate community variation. Stream chemistry was insignificant, with the exception of turbidity. Agricultural activity in the upper and middle catchments caused middle and lower stations to suffer more from the severity of spates and these had a deleterious affect on stream communities. Pollution sensitive fauna were more closely associated with undisturbed stations. Results from a hybrid analysis suggested that a significant variable was not included in the analysis. It is likely that hydrobiological data would have been a significant correlate in explaining macroinvertebrate community variation, as the spate frequency regime has been identified as a significant correlate in other sub/tropical environments.
    Type of Medium: Electronic Resource
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