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  • 1
    ISSN: 1432-1106
    Keywords: Inferior olivary complex ; Cerebellum ; Parasagittal organization ; Autoradiography ; WGA-HRP ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Olivocerebellar organization and topography were analyzed in adult normal (+ / +), heterozygous weaver (wv/+), and homozygous weaver (wv/wv) mutant mice. The two genotypes (wv/+ and wv/wv) of the weaver mutant present a gradation of abnormal cerebellar morphology. Purkinje cell (PC) ectopia ranges from mild (wv/+) to moderate (wv/wv), and regional PC loss is also graded in the two types. To determine olivocerebellar organization and topography, tritiated amino acids were placed into different regions of the inferior olivary complex (IO) in normal, heterozygous, and homozygous weaver mice. Despite some PC loss and ectopia, olivocerebellar fiber (OCF) terminals in both homozygous and heterozygous weaver mice have an orthogonal distribution and topography similar to that seen in normal mice. Differences in OCF termination, such as an increased density of OCF terminal label in the lower portion of the molecular layer, the PC, and granule cell layers, are seen in homozygous weaver mice. In some heterozygous weaver and normal cases, multiple injections labeling most IO cells on one side of the IO resulted in continuous OCF terminal labeling in many regions of the contralateral cerebellar cortex, suggesting that all PCs receive OCF input. Retrograde analysis involving injections of horseradish peroxidase conjugated to wheat germ agglutinin into different mediolateral cerebellar regions in homozygous weaver mice further demonstrates a generally normal olivocerebellar topography.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5060
    Keywords: Allium cepa ; Frankliniella occidentalis(Pergande) ; host plant resistance ; onion thrips ; Thrips tabaci Lindeman ; western flower thrips
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A two season study was conducted to compare the onion cultivars TG1015Y and IPA-3 for resistance to thrips in South Texas. Narrow and broad sense heritabilities were estimated from populations developed from the cross, ‘IPA-3’ × ‘TG1015Y’. Parents, F1, F2, and the respective backcross populations were evaluated for thrips numbers at the Texas Agricultural Experiment Station, Weslaco, TX. ‘IPA-3’ had significantly fewer thrips than ‘TG1015Y’ in both seasons. Yield was significantly different only in the 1996–97 season. The heritability of thrips resistance in this study was very low. Depending on the methods of estimation h 2 was 5.3% and 4.0%, and H 2 was 4.1% and 8.0%. These results suggest that greater genetic gains for thrips resistance in onion can be achieved by selection on a family basis rather than using single plant selection.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1058-8388
    Keywords: Olivocerebellar system ; Development ; Inferior olive ; Cerebellum ; Pattern formation ; Axon outgrowth ; Horseradish peroxidase ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Many projection systems within the peripheral and central nervous system are topographically organized, and it has become increasinging clear that interactions which occur during development determine the projection patterns these systems exhibit in the adult. The olivocerebellar system was chosen as a model system for this study of afferent pattern formation because it has several characteristics which lend themselves to a study of this type. Applications of horseradish peroxidase were made to both the cerebellar primordium and to the inferior olive of embryonic and neonatal mice using an in vitro perfusion system to support the tissue during the transport period. Fibers labeled after restricted olivary applications are limited to particular mediolateral regions of the cerebellum. Similarly, olivary cells retrogradely labeled after discrete cerebellar applications are restricted to particular olivary subdivisions. The results indicate that the olivocerebellar projection displays elements of topographic organization as early as E15 and that the pattern displayed is roughly comparable to that of the adult mammal. The observed trajectories of olivocerebellar fibers and their concomitant association with both Purkinje and cerebellar nuclear cells during embryonic development suggests a role for either or both cell types in the pattern formation process. © 1993 Wiley-Liss, Inc.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1572-9788
    Keywords: Allium cepa ; cytoplasmic male sterility ; DNA diagnostics ; Genetic Bit Analysis ; onion ; single-nucleotide polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Genetic Bit Analysis (GBA) is a relatively new technique developed to score single-nucleotide polymorphisms among alleles. It is currently being used as a genetic diagnostic tool in human paternity tests as well as in pedigree analysis of farm animals. GBA relies on the polymerase chain reaction and enzyme-linked colorimetry to distinguish differences among genotypes. The identification of a single-nucleotide polymorphism which distinguished the plastome of cytoplasmic male-sterile onion varieties from the plastome of fertile lines provided a model system for testing the utility of GBA in plants. Our results demonstrate that GBA permits rapid and accurate allele determination in onion breeding lines, resulting in accurate prediction of sterility at the seedling stage. The use of 96-well microtiter plates and automated liquid-handlers allowed semi-automation of Genetic Bit Analysis.
    Type of Medium: Electronic Resource
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