ISSN:
1573-6849
Keywords:
Allium fistulosum
;
bromodeoxyuridine
;
hetero-chromatin
;
immunodetection
;
replication banding
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Bromodeoxyuridine pulse labelling and immunodetection were applied to synchronized Allium fistulosum cells to study sequential changes in the chromosome replication pattern during S-phase. The replication patterns were classified into five main types depending on the location of the replication signals: (1) over the whole chromosomes; (2) at proximal and interstitial regions; (3) at proximal, interstitial and distal regions; (4) at interstitial and distal regions; and (5) at distal regions. The frequencies of each pattern changed sequentially according to the timing of BrdU incorporation, demonstrating the temporal order of chromosome replication during S-phase. The distal regions that correspond to the major C-bands replicated throughout S-phase except for the earliest stage, but most intensely in late S-phase. The replication time of different chromosome sites overlapped, which is quite different from the biphasic mode of replication tha t occurs in mammalian chromosomes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1009201409644
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