ISSN:
1438-2199
Keywords:
Amino acids
;
Taurine
;
Neurons
;
NCAM
;
Migration
;
Transcription
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Cultures of dissociated cerebellum from 5- to 6-day-old mice as well as of the N2A neuronal cell line were exposed to guanidino ethane sulfonate (GES, 2–5 mM) to reduce the cellular taurine content. Control cultures were kept in culture medium or medium containing 2–5 mM GES plus 2–5 mM taurine to restore the intracellular taurine content. Taurine depletion led to changes in the expression of certain splice variants of NCAM mRNA such as the AAG and the VASE containing forms, while no differences were seen in the expression of the three forms of NCAM protein. In the N2A cells taurine depletion led to a decreased migration rate of the cells. The results suggest that the reduced migration rate of neurons caused by taurine depletion may be correlated to changes in expression of certain adhesion molecules such as NCAM. Moreover, taurine appears to be involved in regulation of transcription processes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01345281
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