ISSN:
1471-4159
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
Abstract: Sprague-Dawley rats (200-260 g) were anesthetized with chJoral hydrate (400 mg/kg) and polyethylene cannulae were permanently implanted into the lateral ventricles. One or two days later, l-buthionine-[S,R]-sulfoximine (L-BSO), an apparently selective inhibitor of γ-glutamylcysteine synthetase, was administered intracerebroventricularly through the cannulae. The brain content of glutathione (GSH) was determined by HPLC with electrochemical detection (gold/ mercury electrode) using N-acetylcysteine as internal standard. A time-course study of the changes in the striatum following a single dose of l-BSO (3.2 mg) revealed a maximal depletion of GSH (-60%) approximately 48 h after the administration. The effects of various doses of l-BSO on GSH in the striatum, in the limbic region, and in the cortex were assessed at 24 h and 48 h after the administration. l-BSO (0.02-3.2 mg) produced dose-dependent reductions of GSH in all brain regions studied at both time intervals. In a long-term experiment l-BSO (3.2 mg) was administered every second day. After 4 days, i.e., after two injections, striatal GSH was reduced by approximately 70%. No further depletion of GSH was obtained by additional injections of l-BSO, but GSH was maintained at this low level for the 12 days studied. These results suggest that l-BSO, administered intracerebroventricularly, would serve as a useful tool for evaluation of the biological role of GSH in the CNS.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1471-4159.1989.tb09256.x
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