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  • Amphibian embryogenesis  (1)
  • Chicken vegetalizing factor  (1)
  • Extracellular material  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Isolation of plasma membranes from Xenopus embryos (1986)
    Springer
    Development genes and evolution 195 (1986), S. 117-122 
    Details
    ISSN: 1432-041X
    Keywords: Plasma membranes ; Amphibian embryogenesis ; Neural induction ; Xenopus laevis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plasma membranes were isolated in high yield from Xenopus gastrulae by repeated sedimentation in discontinuous sucrose gradients. Most of the yolk was separated by lowspeed sedimentation before centrifugation on the discontinuous sucrose gradients. The isolation of plasma membranes was followed by covalent labelling of the surface of dissociated gastrula cells with diazoniobenzene sulphonate, by electron microscopy and the distribution of enzymatic markers. The isolated plasma membranes have a low neural inducing activity as compared to other cell constituents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00456107
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Neural induction in amphibians (1983)
    Springer
    Development genes and evolution 192 (1983), S. 45-47 
    Details
    ISSN: 1432-041X
    Keywords: Neuralizing factor ; Transmission ; Mesoderm ; Extracellular material ; Triturus embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The neural plates of very early neurula stages of Triturus alpestris were removed, the material which is released from the extracellular space between mesoderm and neural plate to the medium in which the embryos were dissected was isolated and extracted with phenol. The protein isolated from the phenol layer showed neural inducting activity. Proteoglycans isolated from the aqueous layer did not show such inducing activity. These results together with previously published experiments (Wilhelm Roux's Arch 184: 285–299) suggest that a neuralizing factor which is released from the mesoderm acts on the inner surface of the overlying dorsal ectoderm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848769
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A mesoderm-inducing factor from a Xenopus laevis cell line (1989)
    Springer
    Development genes and evolution 198 (1989), S. 8-13 
    Details
    ISSN: 1432-041X
    Keywords: Mesoderm induction ; Xenopus XTC factor ; Chicken vegetalizing factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have compared the chemical properties and biological activities of the mesoderm-inducing factor that is secreted by the Xenopus XTC cell line with the vegetalizing factor from chicken embryos. The inducing activity of the factors was tested in different concentrations on totipotent ectoderm either by implantation into early gastrulae of Triturm alpestris or by application of solutions to isolated ectoderm of early gastrulae of Xenopus laevis. Both factors have similar properties. They are not irreversibly inactivated after treatment with 6 M urea or with phenol at 60° C. Reduction with thioglycolic acid inactivates the factors completely. The inducing activity of XTC-conditioned medium decreases only slightly after treatment with 50% formic acid. The apparent molecular mass and the isoelectric point of the factors are similar. The XTC factor was partially purified by size-exclusion and reversed-phase high-pressure liquid chromatography and by isoelectric focusing. The possible relationship of these factors to transforming growth factor β is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00376364
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    Paper (German National Licenses)
    Fulltext
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