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  • 1
    Electronic Resource
    Electronic Resource
    Effect of calcium, furosemide and chlorothiazide on net volume reabsorption and basolateral membrane potential of the distal tubule (1981)
    Springer
    Pflügers Archiv 389 (1981), S. 121-126 
    Details
    ISSN: 1432-2013
    Keywords: Distal renal tubule ; Amphiuma ; Basolateral membrane potential ; Volume reabsorption ; Calcium ; Diuretics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In the distal tubule of the isolated kidney of Amphiuma net volume reabsorption (split-oil droplet method) and basolateral membrane potential (Ψ b ) were measured. Luminal perfusion solution could be changed rapidly from 108 mmol·l−1 NaCl plus 0.1 mmol·l−1 calcium to solutions containing 103 or 97 mmol·l−1 NaCl plus 3.6 or plus 7.2 mmol·l−1 calcium. Furthermore, 10−4 mol·l−1 furosemide or chlorothiazide were applied luminally. (1) Addition of 7.2 mmol·l−1 calcium hyperpolarized Ψ b from −73.4 mV to −108.3 mV and inhibited net volume reabsorption. (2) Similarly, when furosemide was injected, Ψ b was hyperpolarized and net volume reabsorption reduced. Application of both high calcium and furosemide further inhibited volume reabsorption. (3) The effects of chlorothiazide were similar to those of furosemide. However, when both high calcium and chlorothiazide were administered Ψ b and volume reabsorption were almost normalized. (4) The data are consistent with the hypothesis that calcium and the diuretics interfere primarly with chloride uptake across the luminal membrane and thus reduce sodium chloride transport. When chlorothiazide in the presence of high luminal calcium almost normalized chloride transport, it is likely that its effects were by stimulating calcium transport and thus increasing intracellular calcium activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582101
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  • 2
    Electronic Resource
    Electronic Resource
    Intracellular potentials in the isolated human cornea (1974)
    Springer
    Pflügers Archiv 346 (1974), S. 351-360 
    Details
    ISSN: 1432-2013
    Keywords: Isolated Human Cornea ; Intracellular Potentials ; Corneal epithelium ; Ouabain ; Acetazolamide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Intracellular potentials in the epithelium of the isolated human cornea were studied with 3 M KCl-filled microelectrodes. The average potential profile increased slightly and reached a maximal value in a depth of about 40 μ from the epithelial surface. The maximal potentials of 27.4±1.5 m V in a closed chamber of the Ussing-Zerahn type and 45.3±1.7 mV in an open chamber were constant up to 180 min after incubation. Using microelectrodes with high resistance the stromal potentials were close to 0 mV. Intracellular potentials were reduced by about 30% 2h after addition of 10−3 M ouabain/l or 10−3 M acetazolamide/l. A significant inhibition could be obtained 30 min after addition of the drugs. Ouabain in a dose of 10−5 M/l showed no inhibitory effect. The data are consistent with the hypothesis of electrolyte transport systems (Na, Cl, HCO3, H) located in the epithelium of the human cornea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00596190
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The effects of extracellular potassium, ouabain, and prostaglandins on intracellular potassium activity in sheep cardiac Purkinje fibers (1980)
    Springer
    Pflügers Archiv 388 (1980), S. 169-175 
    Details
    ISSN: 1432-2013
    Keywords: Sheep Purkinje fiber ; Intracellular potassium activity ; Extracellular potassium concentration ; Ouabain ; Prostaglandins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract (1) Intracellular K activity (a K i ) of sheep heart Purkinje fibers was measured using K-selective microelectrodes (liquid ion exchanger).a K i in the resting state with an extracellular K of 4 mmol·l−1 was 112.9±6.1 mmol·l−1 (n=47) for a membrane potential (V M) of −73.3±0.9 mV.V M deviated from the calculated potassium equilibrium potential (E K=−93 mV). (2) When extracellular K was decreased to 2 mmol·l−1 or increased to 6 and 10 mmol·l−1 E K changed from −114 to −84 and −73 mV, with little change ina K i . (3)a K i andV M significantly decreased after administration of 10−6 mol·l−1 ouabain. (4) Prostaglandins (PGI2 10–100 μg·l−1 and PGE2 0.01–1 μg·l−1) decreaseda K i without greatly changingV M. The differences betweenV M andE K became smaller. These effects indicate an increase in K permeability and may explain the antiarrhythmic action of prostaglandins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00584124
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  • 4
    Electronic Resource
    Electronic Resource
    Membrane potentials and intracellular chloride activity in the ciliary body of the shark (1986)
    Springer
    Pflügers Archiv 407 (1986), S. S112 
    Details
    ISSN: 1432-2013
    Keywords: Ciliary epithelium ; Shark ; Intracellular membrane potential ; Intracellular chloride activity ; Ouabain ; Furosemide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have found that membrane potential in the isolated ciliary epithelium of the shark,Squalus acanthias, is −53 mV. High extracellular potassium or ouabain (10−5 mol·l−1) decrease the potential, and furosemide (10−4 mol·l−1) hyperpolarizes it. There is no difference in membrane potential between the cells of the non-pigmented and pigmented layers. Intracellular chloride activity (64 mmol·l−1) was significantly higher than could be predicted from the equilibrium distribution (26 mmol·l−1) across the cell membranes. When furosemide was applied to the aqueous side of the epithelium, intracellular chloride activity decreased to 35 mmol·l−1 and approached electrochemical equilibrium. The data indicate that the ciliary epithelium possesses an active, furosemide-sensitive chloride transport mechanism which could be a Na−Cl or a 1 Na-1 K-2 Cl symport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00584939
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    Paper (German National Licenses)
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