ISSN:
1432-203X
Keywords:
Key words ADP-glucose starch glycosyl transferase
;
Amyloplast
;
BY-2
;
Nicotiana tabacum
;
Transcription/translation inhibitors
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract When BY-2 cultured tobacco (Nicotiana tabacum L.) cells were transferred to auxin-depleted culture medium containing cytokinin (benzyladenine, 1 mg/l), the starch content per cell started increasing from 18 h of culture and amyloplasts had formed by 48 h. Pulse-treatment of the cells with actinomycin D and cycloheximide for the first 12 h (or longer) of culture significantly decreased the cellular starch content after 48 h, whereas the starch content did not decrease significantly when the cells were released from the inhibition within 6 h. This suggests that nuclear gene expression necessary for amyloplast formation begins 6–12 h after the transfer. Immunoblotting analysis of the accumulation of ADP-glucose starch glycosyl transferase (starch synthase) supported this inference.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050627
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