ISSN:
0006-3592
Keywords:
Bacillus subtillis
;
binding free energy
;
Adsorption isotherm
;
monolayer adsorption process
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The goal of this work was to characterize the adsorption of Bacillus subtills α-amylase onto crystalline starchy materials of the B-type polymorph. Monodisperse spherulitic particles (R ż6; 5.0 μm), essentially resistant to α-amylolysis at 25°C were prepared from short amylose chains (DPn ≈ 15). The α-amylase adsorbed specifically onto the spherulites, and adsorption was found to be a prerequisite step for hydrolysis. Adsorption was inhibited by the presence of maltose and maltotriose in the reaction mixture. Adsorption isotherm of the enzyme on the particles showed a well developed plateau of 1.62 μg/cm2 at 25°C corresponding to a monolayer adsorption process. The binding free energy calculated from the initial slope of the isotherm was ΔG ≈ -20.7 kJ/mol. This is smaller than published values for the binding of α-amylase to soluble amylosic chains (ΔG 〈 -30 kJ/mol).
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260380204
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