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  • 1
    ISSN: 1432-2242
    Keywords: Complementation ; Granule-bound starch synthase ; Amylose content ; Gene expression ; Inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Granule-bound starch synthase (GBSS) catalyses the synthesis of amylose in starch granules. Transformation of a diploid amylose-free (amf) potato mutant with the gene encoding GBSS leads to the restoration of amylose synthesis. Transformants were obtained which had wild-type levels of both GBSS activity and amylose content. It proved to be difficult to increase the amylose content above that of the wild-type potato by the introduction of additional copies of the wild-type GBSS gene. Staining of starch with iodine was suitable for investigating the degree of expression of the inserted GBSS gene in transgenic amf plants. Of the 19 investigated transformants, four had only red-staining starch in tubers indicating that no complementation of the amf mutation had occured. Fifteen complemented transformants had only blue-staining starch in tubers or tubers of different staining categories (blue, mixed and red), caused either by full or partial expression of the inserted gene. Complementation was also found in the microspores. The segregation of blue- and red-staining microspores was used to analyse the inheritance of the introduced GBSS genes. A comparison of the results from microspore staining and Southern hybridisation indicated that, in three tetraploid transgenics, the gene was probably inserted before (duplex), and in all others after, chromosome doubling (simplex). The partial complementation was not due to methylation of the HPAII/MSPI site in the promoter region. Partially complemented plants had low levels of mRNA as was found when the GBSS expression levels were inhibited by anti-sense technology.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1617-4623
    Keywords: Amylose content ; Antisense RNA ; Dominant (hemizygous) suppression ; Granule-bound starch synthase ; Transgenic potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Granule-bound starch synthase [GBSS; EC 24.1.21] determines the presence of amylose in reserve starches. Potato plants were transformed to produce antisense RNA from a gene construct containing a full-length granule-bound starch synthase cDNA in reverse orientation, fused between the cauliflower mosaic virus 35S promoter and the nopaline synthase terminator. The construct was integrated into the potato genome by Agrobacterium rhizogenes-mediated transformation. Inhibition of GBSS activity in potato tuber starch was found to vary from 70% to 100%. In those cases where total suppression of GBSS activity was found both GBSS protein and amylose were absent, giving rise to tubers containing amylose-free starch. The variable response of the transformed plants indicates that position effects on the integrated sequences might be important. The results clearly demonstrate that in tubers of potato plants which constitutively synthesize antisense RNA the starch composition is altered.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 297 (1979), S. 262-264 
    ISSN: 1618-2650
    Keywords: Analytische Chemie ; Planung für die Hochschulausbildung in den Niederlanden
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary A nationwide planning of Analytical Chemistry in universities should meet two requirements: formulation of a common minimum programme for all universities and a well-balanced spread of research themes over the different universities. In the Netherlands this planning is the object of “ISOAN”, the Intersubfaculty Deliberation on Analytical Chemistry. Perhaps spectacular results have not yet been obtained, however thinking about these subjects has led to a more lucid picture and better mutual understanding.
    Notes: Zusammenfassung Eine solche allgemeine Planung für die Analytische Chemie sollte zwei Forderungen erfüllen: die Aufstellung eines gemeinsamen Mindestprogramms für alle Universitäten und eine ausgewogene Verteilung der Forschungsthemen auf die verschiedenen Hochschulen. In den Niederlanden gehört diese Planung zu den Aufgaben des »ISOAN« (Intersubfakultäts-Beratung über Analytische Chemie). Obwohl bisher keine besonders auffallenden Ergebnisse erzielt wurden, konnte doch durch die Beratungen ein klares Bild der Analytischen Chemie und ein besseres gegenseitiges Verständnis erreicht werden.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 297 (1979), S. 308-312 
    ISSN: 1618-2650
    Keywords: Analytische Chemie ; Ausbildung in den Niederlanden
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary In this paper a survey is presented of the actual situation with regard to the education in Analytical Chemistry at the universities in the Netherlands.
    Notes: Zusammenfassung Ein Überblick wird gegeben über die gegenwärtigen Personal- und Unterrichtsverhältnisse an den verschiedenen Universitäten und Hochschulen des Landes.
    Type of Medium: Electronic Resource
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