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  • 1
    Electronic Resource
    Electronic Resource
    Purification of iron superoxide dismutase from the cyanobacterium Anabaena cylindrica Lemm. and localization of the enzyme in heterocysts by immunogold labeling (1992)
    Springer
    Planta 187 (1992), S. 438-444 
    Details
    ISSN: 1432-2048
    Keywords: Anabaena ; Heterocyst ; Iron-superoxide dismutase (immunogold labeling)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Iron superoxide dismutase (Fe-SOD; EC 1.15.1.1) was isolated from the nitrogen-fixing cyanobacterium Anabaena cylindrica Lemm. Polyacrylamide gel electrophoresis separated the purified protein into three closely running, enzymatically active bands. The molecular weight of the enzyme was estimated by gel filtration to be about 40 kDa. Polyclonal antibodies were produced by immunization of rabbits with the isolated enzyme, and were purified on a column of protein A-Sepharose. The Fe-SOD antibody reacted with the purified Fe-SOD and also specifically recognized the protein in extracts of A. cylindrica. In the extracts, anti-Fe-SOD did not cross-react with Mn-SOD, an enzyme which belongs to an SOD class displaying high homology of primary and three-dimensional structure with respect to Fe-SOD. Iron superoxide dismutase was localized in heterocysts by immunogold labeling and transmission electron microscopy. These results are the first in-situ evidence for the presence of SOD in the cells specialized for nitrogenase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00199961
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  • 2
    Electronic Resource
    Electronic Resource
    Localization of iron-superoxide dismutase in the cyanobiont ofAzolla filiculoides Lam (1992)
    Springer
    Protoplasma 169 (1992), S. 1-8 
    Details
    ISSN: 1615-6102
    Keywords: Immunogold labeling ; Iron-superoxide dismutase ; Cyanobiont ; Azolla filiculoides ; Heterocysts ; Nitrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Immunogold labeling and transmission electron microscopy were used to localize iron-superoxide dismutase (Fe-SOD) in the different cells of nitrogen-fixing cyanobacterial symbiont present within different leaf cavity groups ofAzolla filiculoides Lam. As evidenced by Western blotting and immunoprecipitation, Fe-SOD antibody fromAnabaena cylindrica recognized Fe-SOD in extracts of the cyanobiont and showed the same electrophoretic mobility and pattern as purifiedA. cylindrica Fe-SOD. In vegetative cells of the cyanobiont, Fe-SOD was mainly localized in the thylakoidal membranes and in the outer membrane. The labeling pattern was similar in vegetative cells of the various groups of leaf cavities examined except at the apex where a lower gold particle density was seen. In heterocysts of the leaf cavity groups containing high nitrogenase activity, Fe-SOD labeling was most pronounced and more intense than in vegetative cells. The Fe-SOD label was preferentially located throughout the heterocyst cytoplasm and in the honeycomb regions. In accordance with the decline in nitrogenase activity, the Fe-SOD gold particle density decreased significantly in heterocysts of basal leaf cavity group. The presence of Fe-SOD in regions of high nitrogenase protein levels, and the fact that the pattern of Fe-SOD label parallels that of nitrogenase activity support a role of Fe-SOD in the protection of nitrogenase against superoxide radicals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01343364
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of antibiotic treatments onAzolla-Anabaena andArthrobacter (1991)
    Springer
    Plant and soil 137 (1991), S. 151-155 
    Details
    ISSN: 1573-5036
    Keywords: Anabaena ; Arthrobacter ; Azolla ; erythromycin ; novobiocin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The leaf cavities and the sporocarps of the water fernAzolla contain bacteria belonging to the genusArthrobacter Conn and Dimmick. The number of these bacteria can be decreased by treating the plants with novobiocin and erythromycin. The latter antibiotic kills theAnabaena, soAnabaena free plants can be obtained. In these plants the bacteria disappear almost completely. The novobiocin does not affect the heterocyst differentiation, but it decreases the N2-fixation activity by affecting the synthesis of the dinitrogenase reductase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02187447
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    Paper (German National Licenses)
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