Electronic Resource
Springer
Plant molecular biology
12 (1989), S. 667-672
ISSN:
1573-5028
Keywords:
ferredoxin
;
gene expression
;
heterocyst
;
Anabaena 7120
;
Escherichia coli
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The genes for ferredoxin from heterocysts (fdx H) and vegetative cells (pet F) of Anabaena sp. strain 7120 were subcloned into plasmid pUC 18/19. Both genes were expressed in Escherichia coli at high levels (≈10% of total protein). Pet F could be expressed from its own promoter. The ferredoxins were correctly assembled to the holoprotein. Heterocyst ferredoxin was purified from E. coli extracts on a large scale. Its biochemical and biophysical properties were identical to those of the authentic ferredoxin, isolated from Anabaena heterocysts.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00044157
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