ISSN:
0935-6304
Keywords:
Analysis of high molecular weight compounds
;
Gas chromatography Versus supercritical fluid chromatography
;
Aluminum clad fused silica capillary columns
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Samples, representative of certain classes of “high molecular weight” and/or less volatile substances, were analyzed by gas chromatography utilizing a new generation of aluminum clad flexible fused silica glass capillary columns containing thin films of a methyl polysioxane stationary phase capable of being operated isothermally to 400-425°C and temperature programmed to 425-440°C. The results obtained here were compared with those described in the literature discussing the latest advances in Supercritical Fluid Chromatography (SFC) in the analysis of precisely the same types of samples. In all instances, the gas chromatographic method provided superior speed of analysis, superior column efficiency, and superior resolution of the component bands. This was due to fundamental factors, favoring the diffusivity of the solutes in both the gas and liquid phases in this process at these high temperatures. Under these circumstances, it is thought that the strength of SFC mainly lies in the analysis of thermally labile samples until the advocates of this technique make more definitive strides in the handling of still higher molecular weight substances well beyond the newly expanded range of gas chromatography.
Additional Material:
9 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240091203
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