ISSN:
0935-6304
Keywords:
Micro high performance liquid chromatography, micro-HPLC
;
Resonance Raman detection
;
Dabsylamide
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A micro high performance liquid chromatography coupled with a resonance Raman detection system is described. For highly sensitive Raman detection, aliphatic amines were derivatized with dabsyl chloride (4-dimethylaminoazobenzene-4′-sulfonyl chloride). The derivatives were separated on an ODS micro column (0.5 mm i.d. × 145 mm PTFE tube). Chromatograms were obtained by measuring the intensity of Raman scattering at 1136 cm-1 with the 488.0 nm line of an Arion laser. The lower detection limit was 1.5 ng and the RSD of relative peak height (n = 9) was 5.9% at 11 ng of methylamine derivative. Moreover, by stopping the flow of the micro HPLC system at the retention time of the individual derivatives, it was possible to measure their resonance Raman spectra.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240100403
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