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  • Analytical Chemistry and Spectroscopy  (1)
  • Copy number control  (1)
  • Cross prediction  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 84 (1992), S. 662-671 
    ISSN: 1432-2242
    Keywords: Sugarcane ; Cross prediction ; Univariate normal probability ; Best linear unbiased predictor ; Family selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A quick, accurate method to determine the potential of a sugarcane (Saccharum spp.) cross to produce elite progeny is needed for maximizing genetic gain. Development of a practical cross appraisal method was initiated by evaluating 1,800 progeny from 15 crosses among 23 parents at two intrarow plant spacings (41 cm and 82 cm). Plant spacing was examined for its affect on stool weight variability. The goals were to identify the most reliable and/or easily obtained cross appraisal statistic and to determine the earliest breeding program stage and crop to collect these statistics. Three tests, on plant cane (PC) and first ratoon (FR) single stool seedlings and clonal plant cane plots, were conducted. Four statistics, the family mean, the estimated elite proportion (PROB), the observed elite proportion, and the best linear unbiased predictor (BLUP) were estimated and examined for each cross. These statistics were strongly correlated within each test (0.69≤r≤1.00). Family worth estimates based on single stool data were moderately correlated (ca. range 0.5≤r≤0.7) to the family worth estimates based on clonal plots. The research suggested that the potential of a cross to produce elite progeny for a trait could be accurately predicted by the cross mean of that trait. Data for the mean were the most easily obtained and, hence, would be the most practical family appraisal statistic to use in a breeding program. Correlations of statistics among the PC and FR seedlings and the clonal plots showed that the PC estimates of Brix, stalk weight, and its components, stalk length and stalk diameter, could be used for cross appraisal. Genotypic selection by the Louisiana Sugarcane Variety Development Program (LSVDP) occurs among the FR seedlings. FR stalk number and PC Brix and stalk weight data could be used to perform family selection prior to the currently practiced individual plant selection. The benefits of family selection to the LSVDP were demonstrated by the expected genetic gains for two selection scenarios. The gains were consistently larger for an initial 50% family selection and subsequent 20% individual selection than they were for simple individual selection at a 10% selection intensity. Our research also suggests that the use of a wider intrarow spacing may improve the ability to discern among seedlings due to its enhancement of stool weight variability.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 250 (1996), S. 699-704 
    ISSN: 1617-4623
    Keywords: Key words Iteron ; Plasmid incompatibility ; Copy number control ; Replication protein ; Autoregulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A 1.6-kb DNA region required for the replication of pSW500 from Erwinia stewartii SW2 has been identified. DNA sequencing analysis revealed that this DNA fragment consists of a DnaA box, seven 16-bp direct repeats, and a 1005-bp open reading frame. The seven direct repeats have been demonstrated to mediate the incompatibility function of the plasmid. Primer extension analysis showed that the 1005-bp ORF is transcribed in vivo and the +1 site of the transcript is located 113 bp upstream from the translation initiation codon of the ORF. Complementation studies showed that this ORF is required for the replication of the plasmid and may encode a replication protein, RepA. Gene fusion studies revealed that the expression of repA is autoregulated by RepA. We also found that the pSW500 replicon has a copy number of approximately two and that the plasmid is stably maintained in Escherichia coli, thus demonstrating that the replicon contains all the elements required for copy number control and plasmid stability in E. coli. Curing of pSW500 from E. stewartii SW2 revealed that loss of pSW500 did not have any obvious effect on morphology or physiology of the cells, suggesting that pSW500 does not encode a function that is indispensable for the survival of the organism.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Relationships were found between experimentally measured molecular radical anion abundances and calculated lowest unoccupied molecular orbital energies (εLUMO) for polychlorodibenzofurans and polychlorodibenzo-p-dioxins. Anion abundances were measured using standard using standard mass spectrometric techniques, while εLUMO were calculated by the ‘Complete Neglect of Differential Overlap’ method. Polychlorodibenzofurans with calculated εLUMO ≥ 1.6 eV show 0% molecular radical anion and those with εLUMO ≤ 1.4 eV show ≥ 80% molecular radical anion abundance. Similarly, the molecular radical anion is absent for polychlorodibenzo-p-dioxins with calculated εLUMO ≥ 2.0 eV. A trend towards greater molecular radical ion relative abundance appears for 2.0 eV εLUMO ≥ 1.0 eV and a maximum is reached around 1 eV, whereupon the molecular ion abundance diminishes with lower εLUMO. B/E linked scan analysis indicates that chlorodioxins with εLUMO 〈 1 eV give increasing amounts of metastable anions.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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