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  • Analytical Chemistry and Spectroscopy  (3)
  • Cyanobacteria  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Cloning and expression of a Nostoc sp. leucine biosynthetic gene in Escherichia coli (1986)
    Springer
    Archives of microbiology 145 (1986), S. 315-321 
    Details
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; DNA restriction and cloning ; Gene fusions ; Leucine biosynthesis ; Mutant complementation ; Nostoc ; Transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genomic DNA extracted from the symbiotically-competent, heterocyst-forming cyanobacterium Nostoc sp. strain 7801 was resistant to cleavage by a number of restriction endonucleases. A cosmid library of Nostoc DNA was prepared and maintained in the modification-limited Escherichia coli strain HB101. Analysis of cloned Nostoc DNA fragments indicated infrequent occurrence of restriction endonuclease recognition sites in the Nostoc genome. The Nostoc genomic library was screened for sequences complementing mutations in the E. coli leucine and proline biosynthetic operons. Two cosmids complementing leuB were isolated but none for leuA, leuC, leuD, or proA were detected in 1000 cosmids. A 3.0 kb fragment subcloned from one of the cosmids complemented mutations in leuB when inserted into the HindIII site of pBR322 in either orientation, demonstrating that transcription of leuB originated within the cloned fragment. The cloned fragment also carries a second site capable of initiating transcription of fused antibiotic resistance genes. While transcription of Nostoc DNA sequences did occur in E. coli, unknown barriers must also exist that prevented additional biological complementation of specific E. coli mutations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00470864
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and characterization of Anacystis nidulans R2 mutants affected in nitrate assimilation: Establishment of two new mutant types (1988)
    Springer
    Molecular genetics and genomics 213 (1988), S. 223-228 
    Details
    ISSN: 1617-4623
    Keywords: Cyanobacteria ; Nitrate reductase mutants ; Nitrite reductase mutants ; Regulatory mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Eighteen mutant strains of the unicellular cyanobacterium Anacystis nidulans R2 that are unable to assimilate nitrate have been isolated after transposon Tn901 mutagenesis. Characterization of phenotypes and transformation tests have allowed the distinction of five different mutant types. The mutants exhibiting a nitrate reductase-less phenotype were identified as being affected in previously defined loci, as they could be transformed to the wild type by one of the plasmids pNR12, pNR63 or pNR193, which contain cloned genes of A. nidulans R2 involved in nitrate reduction. The mutations in strains FM2 and FM16 appear to affect two other genes involved in nitrate assimilation. Strain FM2 apparently bears a single mutation which results in both lack of nitrite reductase activity and loss of ammonium-promoted repression of nitrate reductase synthesis. FM16 has a low but significant level of nitrate reductase that is also freed from repression by ammonium, and an increased level of nitrite reductase activity. FM16 exhibited properties which indicate that this mutant strain might also be affected in the transport of nitrate into the cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00339585
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A new surface roughening method for glass capillary columns. Sodium chloride deposition from suspension (1979)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 2 (1979), S. 447-455 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; Surface roughening by direct deposition of suspension particles ; NaCl used ; High reproducibility when coated with polar phases ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A method is described for roughening the surface of glass capillary columns for subsequent coating with polar stationary phases. A suspension of sodium chloride, obtained by addition of a saturated solution of sodium chloride in methanol to 1,1,1-trichloroethane, is passed through the column at velocities of 1-5 cm/s. During passage of the suspension, particles of sodium chloride deposit spontaneously on the column wall. The amount of sodium chloride deposited on the column wall is a function of the volume of the suspension passed through and of the contact time of the suspension and the column wall. Ultimately the amount of sodium chloride per unit surface area approaches a maximum. Columns covered with this maximum amount of sodium chloride were prepared with high reproducibility and coated with a number of polar stationary phases. Various factors that influence the stability of the suspension and the deposition of sodium chloride are discussed and minimum requirements are given. A theoretical model is proposed for the mechanism of deposition of sodium chloride particles on the glass wall.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240020715
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    A study of the electron impact-induced fragmentation of 1,6-diaminohexane (1976)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 11 (1976), S. 995-1001 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The mass spectrometric behaviour of 1,6-diaminohexane has been studied with the help of deuterium labelling in every position of the molecule. Extensive intramolecular exchange reactions between the hydrogen atoms of the amino groups and those of the hydrocarbon chain are responsible for the apparent complexity of the fragmentation mode.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210110912
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    A study of the fragmentation of cyclopentane-1,3-diols and of cyclopentane-1,2,4-triols (1975)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 10 (1975), S. 702-710 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The mass spectra of the two cyclopentane-1,3-diol and of the three cyclopentane-1,2,4-triol stereoisomers have been studied. Extensive deuterium labelling allows the detection of complex processes, especially for the elimination of water from the [M - H2OO]+· and [M]+· ions.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210100904
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    Paper (German National Licenses)
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