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Uromyces viciae-fabae onVicia faba: Scanning electron microscopy of frozen-hydrated material (1982)

Beckett, A. ; Porter, R.

Springer

Protoplasma 111 (1982), S. 28-37

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ISSN: 1615-6102

Keywords: Frozen-hydrated ; Low temperature SEM ; Uredium ultrastructure ; Uromyces viciae-fabae

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary The ultrastructure of uredia, intercellular and intracellular hyphae ofUromyces viciae-fabae onVicia faba has been studied by scanning electron microscopy of frozen-hydrated material. Frozen-hydrated leaves will readily fracture and can be viewed on the cold stage of the microscope at liquid nitrogen temperatures for several hours without visible surface changes occurring. Both fungal and host-plant cells show a high degree of morphological preservation with little or no loss of cell contents and with minimal signs of shrinkage. Intracellular hyphae may be filamentous and branched or short and lobed. They are constricted at the point of entry into the host cell. Intercellular matrix material is well preserved around developing pedicels and urediospores and may act as a lubricant. The technique described is very quick compared with conventional preparatory procedures.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01287644

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A re-examination of the synthesis and some properties of the in vitro metabolite, N-hydroxyphenmetrazineAbbreviation: CPBA = m-chloroperbenzoic acid. (1975)

Coutts, R. T. ; Dawe, R. ; Beckett, A. H.

Chichester : Wiley-Blackwell

Biological Mass Spectrometry 2 (1975), S. 137-141

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ISSN: 1052-9306

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: The reported synthesis of N-hydroxyphenmetrazine from phenmetrazine by the action of m-chloroperbenzoic acid could not be repeated. When the synthetic procedure was modified N-hydroxyphenmetrazine was obtained in low yield. The product had different properties from those claimed previously, but was identical to metabolically produced N-hydroxyphenmetrazine. It was characterized by means of thin-layer chromatography, combined gas chromatography and mass spectrometry, preparation of a t-butyldimethylsilyl derivative and oxidation to a mixture of nitrones. Mass spectrometry was also used to characterize these derivatives.

Additional Material: 1 Ill.