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  • Analytical Chemistry and Spectroscopy  (8)
  • Gynogenesis  (2)
  • IAPS-forskolin  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Mutation of two conserved arginine residues in the glucose transporter GLUT4 supresses transport activity, but not glucose-inhibitable binding of inhibitory ligands (1995)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 353 (1995), S. 36-41 
    Details
    ISSN: 1432-1912
    Keywords: Glucose transport ; IAPS-forskolin ; Insulin-regulated glucose transporter GLUT4
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Two arginine residues (RR333/334) in the conserved GRR motif located in the endofacial loop between helix 8 and 9 of the glucose transporter GLUT4 were substituted for leucine and alanine, respectively. Reconstituted glucose transport activity of the construct (GLUT4-RR333/4LA) expressed in COS-7 or LM(TK-) cells was less than 10% of that of the wild-type GLUT4. In contrast, binding of the inhibitory ligand cytochalasin B and glucose-inhibitable photolabeling with IAPS-forskolin were not significantly affected. Exchange of a histidine residue (H337Q) previously believed to be involved in the binding of inhibitory ligands failed to affect any of the investigated parameters. These data suggest that positive charges in the GRR motif at the cytoplasmic surface of the transporter participate in the conformational changes of the carrier protein during the process of facilitated diffusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00168913
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Mutation of two conserved arginine residues in the glucose transporter GLUT4 supresses transport activity, but not glucose-inhibitable binding of inhibitory ligands (1995)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 353 (1995), S. 36-41 
    Details
    ISSN: 1432-1912
    Keywords: Key words Glucose transport ; IAPS-forskolin ; Insulin-regulated glucose transporter GLUT4
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Two arginine residues (RR333/334) in the conserved GRR motif located in the endofacial loop between helix 8 and 9 of the glucose transporter GLUT4 were substituted for leucine and alanine, respectively. Reconstituted glucose transport activity of the construct (GLUT4-RR333/4LA) expressed in COS-7 or LM(TK–) cells was less than 10% of that of the wild-type GLUT4. In contrast, binding of the inhibitory ligand cytochalasin B and glucose-inhibitable photolabeling with IAPS-forskolin were not significantly affected. Exchange of a histidine residue (H337Q) previously believed to be involved in the binding of inhibitory ligands failed to affect any of the investigated parameters. These data suggest that positive charges in the GRR motif at the cytoplasmic surface of the transporter participate in the conformational changes of the carrier protein during the process of facilitated diffusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00168913
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Sex determination in the genus Oreochromis (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 144-152 
    Details
    ISSN: 1432-2242
    Keywords: Sex determination ; Oreochromis niloticus ; Gynogenesis ; Sex reversal ; Triploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Established techniques of genetic manipulation were used to elucidate sex-determining mechanisms in the commercially important tilapia, Oreochromis niloticus. Analysis of sex ratios from single-pair matings of normal broodstock showed these to be heterogeneous, with an asymmetrical frequency distribution. Data were homogeneous, with the exclusion of a number of broods with sex ratios not significantly different from 3∶1 (male: female), and further progeny testing revealed atypical female heterogamety in the parents of these broods. Analysis of sex ratios from complete diallele-type crosses using five males and five females demonstrated no association between male parent, female parent and progeny sex ratio. Sex ratios of gynogens (0∶1) and triploids (1∶1), and from progeny testing of sex-reversed males (0∶1) and sex-reversed females (3∶1), provide evidence for female homogamety in this species. Progeny testing of male gynogens derived from sex-reversed females demonstrated recombination between the centromere and the sex-determining locus (68.9%). Novel YY “supermales” were shown to be viable and produced all-male offspring. It was concluded that this species exhibits monofactorial, genotypic sex determination with male heterogamety. However, rare autosomal or environmental sex-modifying factors may account for occasional deviations from expected sex ratios.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226205
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  • 4
    Electronic Resource
    Electronic Resource
    Sex determination in the genus Oreochromis (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 153-160 
    Details
    ISSN: 1432-2242
    Keywords: Sex determination ; Oreochromis aureus ; Gynogenesis ; Sex reversal ; Hybridisation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sex ratios from 62 single-pair matings of normal broodstock O. aureus were highly heterogeneous with an overall deficit of males (41.4%). Peaks in the sex ratio frequency distribution occurred at 1∶1, 3∶5 and 1∶3 (male∶female). Hybridisation of O. aureus with O. mossambicus, O. spilums and O. niloticus produced highly variable sex ratios, suggesting a complexity of hybrid sex determination. Few valid inferences could be made regarding intraspecific sex determination from these hybrid data. Sex ratios from progeny testing of sex-reversed males (1∶3) and most sex-reversed females (1∶0) provide evidence for female heterogamety in O. aureus. Several aberrant ratios observed suggest Mendelian inheritance of an autosomal recessive gene (F,f), epistatic to the major sex-determining gene (W,Z). Sex ratios of triploids and gynogens support the hypothesis of recombination between the centromere and the major sex-determining locus. Progeny testing of a female mitogyne demonstrated the viability of a novel WW “superfemale”, which gave only female offspring. Not all data could be explained by a two-factor model of sex determination. Further exceptional sex ratios may be accounted for by rare autosomal or environmental sex-modifying factors. It is concluded that O. aureus has a multifactorial mechanism of sex determination with the underlying primary mechanism of female heterogamety.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226206
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Capillary electrophoresis/electrospray ionization ion trap mass spectrometry using a sheathless interface (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Microcolumn Separations 7 (1995), S. 461-469 
    Details
    ISSN: 1040-7685
    Keywords: mass spectrometry ; on-line capillary electrophoresis ; MS / MS analysis ; sheathless electrospray source ; peptides ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The sensitivity that can be obtained with the quadrupole ion trap for on-line CE analysis using a sheathless electrospray interface and gold-tipped columns is examined. Capillaries of different i.d. are compared in continuous infusion experiments and the effects of sheath solvents on the signal for some peptides and proteins are illustrated. The sheathless CE/ITMS system using 20 μm i.d. columns is shown to provide mid-attomole detection levels for leucine enkephalin in total ion electropherograms under full scan conditions. Good quality MS/MS spectra are also obtained for low femtomole injections of this peptide.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mcs.1220070505
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  • 6
    Electronic Resource
    Electronic Resource
    Simultaneous determination of the electroosmotic flow and pseudo-stationary phase migration time in electrokinetic capillary chromatography (1995)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 18 (1995), S. 439-442 
    Details
    ISSN: 0935-6304
    Keywords: Electrokinetic chromatography (EKC) ; Pseudo-stationary phase ; Electroosmotic flow ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240180709
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  • 7
    Electronic Resource
    Electronic Resource
    Ion-trap mass spectrometry with an inductively coupled plasma sourcePaper presented in part at the 41st ASMS Conference on Mass Spectrometry and Allied Topics, 30 May-4 June, 1993, San Francisco, CA, USA. (1994)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 8 (1994), S. 71-76 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: A new mass spectrometry technique using an inductively coupled plasma (ICP) ionization source and an ion-trap mass spectrometer (ITMS) detector is described and evaluated for elemental and isotopic analysis. Initial results obtained with the ICP-ITMS technique include sub-ppb detection of bare ions for, most elements, complete destruction of typical polyatomic matrix ions (e.g., CIO+, ArO+, and ArCl+), and effective neutralization of strong Ar+ ion currents. The latter effects allow observation of clearer mass spectra at m/z 〈 80 and potentially improved detection of ‘problem’ elements in ICP-MS (e.g., K, Ca, V, Fe, As, and Se). Formation of poly-oxide and -hydroxide ions of metals with high oxide bond strengths were observed and require system modifications to alleviate.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290080114
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  • 8
    Electronic Resource
    Electronic Resource
    Charged vs. neutral nucleobase loss from multiply charged oligonucleotide anions (1995)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 30 (1995), S. 1222-1229 
    Details
    ISSN: 1076-5174
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Tandem mass spectra of a variety of adenine-containing deoxynucleic acid anions obtained using collisional activation in a quadrupole ion trap are described. Data are reported for singly charged mono-, di- and trinucleotide species, and also for multiply charged anions derived from tri-, tetra-, penta- and hexanucleotides. Attention is focused on the first step in the unimolecular decomposition of these species, namely the loss of the nucleobase. Specifically, the competition between the loss of the nucleobase as a neutral versus its loss as an anion is addressed within the context of a proposed proton-bound intermediate consisting of the nucleobase and a phosphodiester linkage. The data are interpreted on the basis of the energy surface involved in the break-up of the proton-bound intermediate, which can explain why the competition between loss of a neutral versus charged base is strongly dependent upon the charge of the parent ion.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jms.1190300903
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  • 9
    Electronic Resource
    Electronic Resource
    Analyzer scan modes for hybrid mass spectrometers (1989)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 24 (1989), S. 470-478 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The scan modes that can be used to obtain daughter ion, parent ion, and constant neutral-loss spectra on hybrid mass spectrometers are derived for instruments with a quadrupole combined with a magnetic and/or electric sector. All combinations of sector/quadrupole and quadrupole/sector instruments are discussed. The advantages or lack thereof of the various scans are presented with respect to operation, resolution and potential artifacts.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210240706
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  • 10
    Electronic Resource
    Electronic Resource
    The effect of charge on hydroxyl loss from ortho-substituted nitrobenzene ions (1987)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 22 (1987), S. 224-228 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An abundant loss of hydroxyl in decompositions of ortho-substituted nitroarene cations is commonly observed when the substituent contains one or more labile hydrogen atoms. The major loss of hydroxyl also takes place from many but not all of the corresponding molecular anions. Data are reported for the collisionally activated decompositions of the cations and anions of o-nitrotoiuene, o-nitrophenol and o-nitroaniline. Data for some dinitro ions are also reported. The results can be rationalized on the basis of a greater degree of charge developed at the substituent in the transition state of the anions that leads to a rearranged ion. It is from this structure tint hydroxyl is lost via simple bond cleavage. This can be viewed most simply as a proton transfer from the substituent to the nitro group in the anion as opposed to hydrogen transfer in the analogous step for the cation. The degree to which hydroxyl loss occurs is therefore largely determined by the tendency for hydrogen (cations), or proton (anions), transfer from the substituent to the nitro group.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210220409
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