ISSN:
0003-276X
Keywords:
Mitochondria
;
Helical filaments
;
Hepatocytes
;
Ethanol
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
Background: Packets of helical filaments have been observed in the outer compartment of occasional mitochondria in many cell types in a variety of animals. The compositon and funcation of these intramitochondrial helical filaments (IMHF) are unknown.Methods: IMHF wre induced in a hepatic mitochondria by administration of ethanol in the drinking water of rats. Hepatic mitochondria were isolated and ruptured by osmotic shock, relasing their IMHF. To purify these structures, the IMHF-containing supernatant was further fractionated by ammonium sulfate precipitation, a 50-60% solution of this reagent being the most effective in this regard. Isolated IMHF were examined by electron microcopy and were analyzed by SDS-PAGE.Result Isolated IMHF closely resembled their in situ counterparts: they had a right-handed helical structure with a 16 nm pitch. SDS-PAGE analysis revealed that they contained three polypeptides with weigh tmolecular of 135, 98, and 53 kD, respectively.Conculusions: These observations will stand as a aseline for comparisons with IMHF that occur naturally or that are induced in other cell types by other kinds of experimental manipulation. © 1995 Wiley-Liss, Inc.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/ar.1092410202
Permalink
