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  • Analytical Chemistry and Spectroscopy  (3)
  • Life Sciences  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Identification of chlorophyll transformation products in zooplankton faecal pellets and marine sediment extracts by liquid chromatography/mass spectrometry atmospheric pressure chemical ionization (1995)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 9 (1995), S. 1177-1183 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Liquid chromatography/mass spectrometry with an atmospheric pressure chemical ionization interface permits the routine study of mixtures of algal-derived, chlorophyll transformation products isolated from biological and environmental samples. Interpretation of the mass spectra of standards of chlorophyll a and three of its commonly occurring transformation products has allowed the identification of components found in extracts from zooplankton faecal pellets and a marine surface sediment. This highly sensitive technique should prove valuable for pigment analysis in environmental studies.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290091218
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    ATP-stimulated transmitter release and cyclic amp synthesis in isolated chromaffin granules (1976)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 4 (1976), S. 181-184 
    Details
    ISSN: 0091-7419
    Keywords: Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: ATP stimulates chromaffin granules from the bovine adrenal medulla to release epinephrine and specific soluble proteins. ATP analogs substituted in the β-γ-position with either nitrogen or carbon were also found to be effective at inducing release from isolated chromaffin granules. However, an ATP analog substituted at the α-β position with carbon was strongly inhibitory. Cyclic AMP was also found to be synthesized by isolated chromaffin granules under release conditions. ATP analogs were effective as substrates for adenylate cyclase in the same order as their efficiency for inducing release from vesicles. Hydrolysis at the β-γ linkage of ATP therefore is probably not necessary for release; however, hydrolysis at the α-β position may be important in the release process. Cyclic AMP may be produced and play a regulatory role in this event.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400040205
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Reversal of order of chemical shifts. Differential solvent effects in the hydrogen shifts of toluene, m-xylene and o-xylene as determined by 320 MHz tritium NMR spectroscopy (1984)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 22 (1984), S. 665-667 
    Details
    ISSN: 0030-4921
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Chemical shifts for the aromatic tritons of toluene decrease in the order meta〉para〉ortho for pure toluene, but in the order meta〉ortho〉para for dilute solutions of toluene in carbon tetrachloride, chloroform, cyclohexane and dimethyl sulphoxide; m-xylene shifts are 5〉4,6〉2 in the pure state and 5〉2〉4,6 in carbon tetrachloride and dimethyl sulphoxide; o-xylene shifts are 4,5〉3,6 in the pure state and reversed in carbon tetrachloride.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1270221014
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Functional conservation of cytosolic proteins required for endosomal vesicle fusion (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 12 (1996), S. 1251-1262 
    Details
    ISSN: 0749-503X
    Keywords: yeast ; Sec18 ; endocytosis ; NEM ; Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Recent studies suggest that intracellular membrane traffic relies upon families of related proteins which confer specificity to individual transport reactions but which operate in tandem with a ubiquitous fusogenic complex containing the N-ethylmaleimide-sensitive fusion protein (NSF). The extent to which components of this process are functionally conserved is apparent from the finding that yeast Sec18 protein (Sec18p) can substitute for mammalian NSF in intra-Golgi transport reactions. Here we report that yeast cytosol can support mammalian endosomal vesicle fusion, demonstrating conservation of cytosolic components required for this reaction. Furthermore, under conditions in which the fusion reaction is NSF-dependent we show that yeast Sec18p can functionally substitute for NSF, showing that the yeast protein is capable of catalysing at least two distinct mammalian membrane fusion events. In addition we exploit the complex pattern of sensitivity of the mammalian reaction to N-ethylmaleimide (NEM), coupled with the use of yeast cytosol, to dissect a number of factors required for fusion. We reveal at least three novel NEM-sensitive activities. One of these can be restored by yeast cytosol suggesting that it is functionally conserved.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
  • 5
    Electronic Resource
    Electronic Resource
    Direct determination of proton-exchange kinetics by 320-MHz tritium NMR spectroscopy (1991)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 29 (1991), S. 509-512 
    Details
    ISSN: 0749-1581
    Keywords: 3H NMR ; Exchange rates ; Kinetic acidity ; Kinetics ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The first application of tritium NMR spectroscopy to the direct observation of proton-exchange kinetics is described; this technique allows the study of milligram amounts of substrates and for substrates with multiple exchangeable positions. The examples include benzene and the ring and methyl positions of p-xylene reacting with cesium cyclohexylamide in cyclohexylamine, and the enolate positions of cyclohexanone and methyl 7,12-dihydroxy-3-ketocholan-24-oate reacting with heptafluorobutyric acid in dioxane.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260290517
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    Paper (German National Licenses)
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