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  • Analytical Chemistry and Spectroscopy  (2)
  • Small intestine  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Subcellular distribution of rat pituitary homogenates by poly(ethylene glycol)-dextran countercurrent partitioning (1986)
    New York, NY : Wiley-Blackwell
    Biomedical Chromatography 1 (1986), S. 12-14 
    Details
    ISSN: 0269-3879
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Rat pituitary homogenates were subjected to two phase countercurrent partition in a poly(ethylene glycol)-dextran mixture using a simple apparatus with enhanced gravity to facilitate the phase separations. Assay of the fractions for organelle marker enzymes and prolactin after 17 transfers showed similar distributions for endoplasmic reticulum, lysosomes, prolactin granules and plasma membrane at the lowest dextran concentrations. Increasing the dextran concentrations had a differential effect on the various organelles. Excellent resolution of endoplasmic reticulum from the other organelles was obtained and marked organelle heterogeneity was demonstrated. Two-phase countercurrent partition thus offers an alternative approach to the subcellular fractionation of pituitary homogenates and should prove useful in separating endoplasmic reticulum from plasma membrane and other cell components.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bmc.1130010104
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    An HPLC assay for rat liver ferrochelatase activity (1987)
    New York, NY : Wiley-Blackwell
    Biomedical Chromatography 2 (1987), S. 164-168 
    Details
    ISSN: 0269-3879
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A rapid, reliable, sensitive and reproducible HPLC method was developed for the assay of ferrochelatase activity in rat liver. The assay was carried out aerobically with Zn2+ and mesoporphyrin or protoporphyrin IX as substrates. Zn-porphyrins formed were extracted with dimethyl sulphoxide/methanol (30:70, v/v) containing Zn-deuteroporphyrin as the internal standard for separation and quantification by reversed-phase chromatography. The Km for mesoporphyrin was 5.9 μM, for protoporphyrin IX 8.8 μM and for zinc 6.0 μM. The specific activities were 33.1 ± 5.0 nmol Zn-mesoporphyrin or 13.4 ± 2.0 nmol Zn-protoporphyrin formed per hour per mg of protein for mitochondria and 12.3 ± 2.2 nmol Zn-mesoporphyrin or 4.6 ± 0.9 nmol Zn-protoporphyrin per hour per mg of protein for liver homogenate.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bmc.1130020408
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Immunofluorescent localization of transglutaminase in rat small intestine (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 6 (1988), S. 137-141 
    Details
    ISSN: 0263-6484
    Keywords: Small intestine ; transglutaminase ; immunohistochemistry ; coeliac disease ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The distribution of intestinal transglutaminase was investigated by immunofluorescence microscopy using rabbit anti-guinea pig transglutaminase immunoglobulin. Transglutaminase-related antigen was demonstrated principally in the cytoplasm of villous core interstitial cells with some activity in the brush border region of the villous epithelial cells. Implications for the pathogenesis of coeliac disease are discussed.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cbf.290060209
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    Paper (German National Licenses)
    Fulltext
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