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  • Analytical Chemistry and Spectroscopy  (2)
  • breast diseases  (1)
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Material
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Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Factors associated with obtaining nipple aspirate fluid: Analysis of 1428 women and literature review (1990)
    Springer
    Breast cancer research and treatment 15 (1990), S. 39-51 
    Details
    ISSN: 1573-7217
    Keywords: age ; breast fluid ; breast diseases ; breast feeding ; breast secretion ; multivariate analysis ; race ; reproductive history ; selection bias
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Studies of cytologic and biochemical constituents of nipple aspirates of breast fluid have contributed to understanding the natural history of benign and malignant breast disease. We conducted multivariate analyses using 1428 women from a recent case-control study of breast disease to determine which factors were independently associated with the ability to obtain breast fluid from nonlactating women. We then compared results from these analyses to the results from five previous studies that also used the aspiration technique of Sartorius. Four factors were consistently associated across studies with increased ability to obtain breast fluid: 1) age up to 35 to 50 years; 2) earlier age at menarche; 3) non-Asian compared to Asian ethnicity; and 4) history of lactation. Exogenous estrogen use, endogenous estrogen concentrations, phase of menstrual cycle, family history of breast cancer, type of menopause, and less than full-term pregnancy consistently did not influence ability to obtain fluid. New findings from this study shed light on some apparently contradictory findings from the previous studies. In particular, this study showed that the effects of age on ability to obtain fluid appeared to be independent of the effects of menopause. Furthermore, discrepancies in previous findings on the effects of parity on ability to obtain fluid may be explained by our finding that the increased ability to obtain fluid from parous compared to nulliparous women applied only to parous women who had breastfed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01811888
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A gas chromatographic mass spectrometric method for the analysis of trifluoroacetic acid: Application to the metabolism of halothane by in vitro preparations (1988)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 17 (1988), S. 471-475 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A selected ion monitoring gas chromatographic mass spectrometric assay for trifluoroacetic acid was developed for the study of the metabolism of the volatile anesthetic agent halothane by in vitro preparations. The assay uses a headspace sampling technique after formation of methyl esters with dimethyl sulfate and sulfuric acid. Pentafluoropropionic acid proved to be a suitable internal standard, although care is required in the preparation of the calibration standards so that they reflect the composition and treatment of the samples. Contamination of the samples, possibly with trifluoroacetic acid itself, was found to be the primary factor in limiting the sensitivity of the assay for the metabolism of halothane. Generally, trifluoroacetic acid could be determined at levels as low as 1 μM in 50-100 μl of incubate.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200170610
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A stable isotope technique for investigating lactate metabolism in humans (1983)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 10 (1983), S. 458-462 
    Details
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A stable isotope tracer method has been developed for studying lactate metabolism in humans. The method uses lactic acid triply labeled with 13C as the tracer. The stable isotope is infused to attain a level of approximately 1.5% of that of the circulating unlabeled lactate. Following the isolation of lactic acid from the blood, the percentage of triply labeled (13C)lactate is measured using gas chromatography mass spectrometry. We compared this method with tracer methodology using [14C]lactate and found comparable results.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200100804
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    Paper (German National Licenses)
    Fulltext
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