ISSN:
0935-6304
Keywords:
Capillary gas chromatography
;
Plasma
;
Verapamil
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A specific, sensitive, and accurate assay for quantitation of verapamil has been developed. The method involves a single extraction step with n-heptane, followed by evaporation at room temperature under nitrogen. 0.1 μl of the extract was injected into a capillary column coated with crosslinked 5% phenylmethylsilicone. The column separated verapamil, norverapamil, and its internal standard within 15 minutes using temperature programming from 90 to 290°C. The lower limit of detection was 1 ng/ml for verapamil. The calibration curve was linear in the concentration range (5-300 ng/ml). Plasma concentration data from dogs receiving intravenous verapamil infusion are presented.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240110126
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