ISSN:
0935-6304
Keywords:
Allergen
;
Wasp venom
;
Phleum pratense and Betula verrucosa pollen
;
Capillary electrophoresis
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A CE method has been developed for the analysis of wasp venoms and pollen extracts of Phleum pratense and Betula verrucosa. Various electrolyte systems can be used but the best separation and reproducibility are attained in 0.15 mol/l phosphoric acid, pH 1.8. UV photometric detection at 190 nm is sufficiently sensitive for determination of the polypeptides and glycoproteins contained in these materials (the detection limit for phospholipase A2 is 0.4 ng). The allergens from wasp venoms and pollen extracts yield characteristic electropherograms which can be used for control of the allergen production. Modification of capillary wall with poly(ethylhydroxy methacrylate) causes a decrease in the migration times and the separation efficiency is retained.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240180914
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