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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 20 (1992), S. 809-819 
    ISSN: 1573-5028
    Keywords: Branching enzyme ; cassava ; cDNA ; expression pattern ; Manihot esculenta Crantz ; sequence homology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Branching enzyme is involved in the synthesis of amylopectin in plant reserve starch. A cDNA coding for cassava (Manihot esculenta Crantz) branching enzyme was cloned from a λgt11 cDNA library using a potato cDNA probe. The cloned cDNA was partially sequenced. The sequence data confirmed the identity of the clone when compared to that of potato, the homology being ca. 80% at the nucleotide level and 85% at the amino acid level. Furthermore, the cloned cassava cDNA was able to restore branching enzyme activity in a branching enzyme deficient Escherichia coli mutant. Results of the Southern analysis suggested that there is a single gene for this particular branching enzyme in the cassava genome. Study of expression patterns by northern hybridization showed that the gene is highly expressed in tubers. The transcript is detectable in stem and petiole, but not in leaves. In roots, the mRNA is hardly present. The expression levels at different stages of tuber growth are similar with exception of very young tubers in which it is relatively low. It is also shown that there is a difference in the level of branching enzyme expression between different cassava genotypes.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 297 (1979), S. 262-264 
    ISSN: 1618-2650
    Keywords: Analytische Chemie ; Planung für die Hochschulausbildung in den Niederlanden
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary A nationwide planning of Analytical Chemistry in universities should meet two requirements: formulation of a common minimum programme for all universities and a well-balanced spread of research themes over the different universities. In the Netherlands this planning is the object of “ISOAN”, the Intersubfaculty Deliberation on Analytical Chemistry. Perhaps spectacular results have not yet been obtained, however thinking about these subjects has led to a more lucid picture and better mutual understanding.
    Notes: Zusammenfassung Eine solche allgemeine Planung für die Analytische Chemie sollte zwei Forderungen erfüllen: die Aufstellung eines gemeinsamen Mindestprogramms für alle Universitäten und eine ausgewogene Verteilung der Forschungsthemen auf die verschiedenen Hochschulen. In den Niederlanden gehört diese Planung zu den Aufgaben des »ISOAN« (Intersubfakultäts-Beratung über Analytische Chemie). Obwohl bisher keine besonders auffallenden Ergebnisse erzielt wurden, konnte doch durch die Beratungen ein klares Bild der Analytischen Chemie und ein besseres gegenseitiges Verständnis erreicht werden.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 297 (1979), S. 308-312 
    ISSN: 1618-2650
    Keywords: Analytische Chemie ; Ausbildung in den Niederlanden
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary In this paper a survey is presented of the actual situation with regard to the education in Analytical Chemistry at the universities in the Netherlands.
    Notes: Zusammenfassung Ein Überblick wird gegeben über die gegenwärtigen Personal- und Unterrichtsverhältnisse an den verschiedenen Universitäten und Hochschulen des Landes.
    Type of Medium: Electronic Resource
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