ISSN:
1432-0428
Keywords:
Anoxia
;
glucose metabolism
;
pancreatic islets
;
Pasteur effect
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary When equilibrated with O2-CO2 (95:5), pancreatic islets of non-inbredob/ob-mice exhibited a sigmoidal dependence of3H2O production on D-(5-3H)-glucose concentration; the rate was most sensitive to changes of glucose concentration around 5mM and tended to be maximum above about 15 mM glucose.3H2O production from more than 5 mM D-(5-3H)-glucose was about twice as fast as the production of14CO2 from equimolar D-(U-14C)-glucose. Islets equilibrated with N2-CO2 (95:5) did not exhibit a sigmoidal dose-response curve for3H2O production, the process being inhibited by anoxia at glucose concentrations above 5mM. Pieces of exocrine pancreas had a slower aerobic3H2O production than the islets and showed a clear enhancement of the process during anoxia. In comparison with oxygenated islets, anoxic islets exhibited decreased concentrations of glucose-6-phosphate and increased concentrations of fructose-l,6-diphosphate. The concomitant inhibition of glycolytic flux may be due to a low lactate dehydrogenase activity in islets yielding a slow reoxidation of NADH and a slow phosphoglyceraldehyde oxidation under anaerobic conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01222098
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