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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 75 (1995), S. 33-42 
    ISSN: 1570-7458
    Keywords: Lyctocoris campestris ; Anthocoridae ; Heteroptera ; seasonal abundance ; trapping efficiency ; disersion pattern ; stored-product ; predator ; sex-ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A two-year sampling study (1992–93) in a flat storage of shelled corn showed that the larger pirate bugLyctocoris campestris (F.) (Heteroptera: Anthocoridae), can be detected by relative sampling techniques as early as the second week of May. The perceived seasonal pattern ofL. campestris varied with trapping method. Of the three relative sampling methods used, probe traps consistently captured more bugs of both sexes than cardboard traps in the grain and sticky flight traps. Probe traps showed a third degree polynomial trend through the season. On the other hand, flight traps were effective for monitoringL. campestris population during early and mid-summer when temperatures were high, but the effectiveness declined during late summer and early fall. Cardboard traps showed the opposite trend to those of flight traps, with increasingL. campestris abundance during the fall when bugs sought refuge as temperature decreased. Sampling date and environmental factors, including grain moisture content, air and grain temperatures were correlated with trap capture. Earlier work reported thatL. campestris showed a highly female biased sex ratio in the field as opposed to a 1∶1 sex ratio in laboratory colonies. The present study found that the estimate of sex ratios in the field can differ with trapping methods. Of the three relative sampling methods used, flight and cardboard traps, in most cases, showed no deviation from a 1∶1, sex ratio. Probe traps, however, indicated a highly female biased sex ratio in field populations. Absolute sampling using a 1140 ml cup sampler also showed no deviation from a 1∶1 sex ratio in the field population. The data suggested that relative sampling methods may be inappropriate for sex ratio estimation in the field. Thus, an absolute sampling method is required for determination of the true field sex ratio ofL. campestris. Analyses of dispersion patterns showed that both males and females ofL. campestris exhibit an aggregated spatial distribution in the grain.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Digestive diseases and sciences 38 (1993), S. 1046-1054 
    ISSN: 1573-2568
    Keywords: mucus ; HT29 ; cholinergic ; calcium ; protein kinase C ; exocytosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Cholinergic stimulation of the HT29-18N2 goblet cell line increased mucin secretion as assessed: (1) with a mucin-specific immunoassay, (2) using whole-mount immunocytochemistry, or (3) by morphometric quantification of intracellular mucous granule stores. Cholinergic stimulation did not, however, result in the apical plasmalemmal membrane cavitation that is characteristic of recent compound exocytotic activity. The resposse was not dependent on protein kinase C activation since it was not inhibited by the kinase C antagonist H7 or potentiated by the diaacylglycerol kinase antagonist R59022. Calcium ionophore A23187 also accelerated mucin secretion by a noncompound exocytotic pathway. Activation of protein kinase C by phorbol 12-myristate 13-acetate, on the other hand, increased mucin secretion by a compound exocytotic pathway. The results provide insight into the signal transduction pathways underlying secretory responses of goblet cells observedin situ.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Digestive diseases and sciences 38 (1993), S. 2299-2304 
    ISSN: 1573-2568
    Keywords: goblet cells ; mucin ; mucus secretion ; cholinergic ; secretion ; morphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Computer-assisted morphometric analysis was used to quantify mucous granule discharge and the subsequent replenishment of secretory granules in the rat colon following cholinergic challenge. Within 5 min of stimulation (250 μg/kg carbachol, subcutaneous), the volume of intracellular mucous granules decreased to 61.5% of the control. The apical plasma membranes of goblet cells in the midcrypt region became deeply cavitated, indicating that they had accelerated mucous granule secretion by a process of compound exocytosis. Goblet cells at the base of the crypt rarely showed cavitated apical membranes but clearly became depleted of intracellular mucous granules. At 60 min after stimulation, cavitated profiles were very rare (≤0.4%) but intracellular stores of mucous granules were still significantly depressed (54.7% of control). By 4 hr after stimulation, mucous granule stores recovered to 94.9% of control levels. Morphometric quantification was found to be a reliable and sensitive measure of recent goblet cell secretory activity.
    Type of Medium: Electronic Resource
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