ISSN:
0935-6304
Keywords:
Fast high-performance liquid chromatography
;
Plasma and urine analysis
;
Solid-phase extraction
;
β-Glucuronide conjugation
;
Anti-brain-edema agent
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A simple and rapid high-performance liquid chromatographic method for the determination of R[(5,6-Dichloro-2,3,9,9a-tetrahydro-3-oxo-9a-propyl-1-H-fluoren-7-yl)oxy]acetic acid (I) in human plasma and urine is described. The method utilizes Bond-Elut® cartridge facilitate the drug extraction. Analysis is performed on a short reversed-phase column with a mobile phase consisting of acetonitrile and phosphate buffer and quantification is carried out by ultraviolet detection with a wave-length set at 340 nm. The method is linear and reproducible for both plasma and urine analyses (0.25-50) μg/mL) with the detection limit of 125 ng/mL of plasma and urine. Plasma and urine concentrations of / at selected time intervals following I.V. administration of single rising doses are presented.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240090705
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