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  • Neurosecretory pathways  (3)
  • Neurosecretory material  (2)
  • Anti-oxytocinase serum  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Localization of neurophysin in the neurosecretory elements of the hypothalamus and neurohypophysis of the normal and osmotically stimulated guinea-pig as demonstrated by immunofluorescence histochemical techniques (1973)
    Springer
    Cell & tissue research 145 (1973), S. 39-55 
    Details
    ISSN: 1432-0878
    Keywords: Magnocellular hypothalamic nuclei ; Accessory structures ; Neurosecretory pathways ; Neurophysin-like material ; Immunofluorescence histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Antisera to porcine neurophysin-II and ovine neurophysin-III were used to localize neurophysin-like material in the hypothalamus and neurohypophysis of guinea-pigs with an immunofluorescence technique. Although the guinea-pig appears to have only one major neurophysin it was found to be localized in both of the bilateral magnocellular nuclei. Neurophysin-like material was also present in extreme rostral portions of the hypothalamus, in cells lying between the third ventricle and the supraoptic nucleus and in a cluster of cells dorsomedial to the fornix. Immunofluorescence was observed in neurosecretory fibres that followed pathways previously characterized with classical histological stains for “neurosecretory material”. The immunofluorescence in the hypothalamic elements of a normal guinea-pig was not greatly different from that in fluorescent structures present in an animal that had been severely dehydrated. In contrast, there was a marked depletion of neurophysin from the posterior pituitary gland of the dehydrated guinea-pig. The lack of graded fluorescence in the hypothalamus of the dehydrated animal is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307188
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Neurophysin and the neurosecretory fibres of the sheep infundibulum (1973)
    Springer
    Cell & tissue research 145 (1973), S. 471-478 
    Details
    ISSN: 1432-0878
    Keywords: Neurophysins (sheep) ; Neurosecretory pathways ; Proximal and distal neurohypophysis ; Infundibulum ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A cross-species reactive antiserum was used to study the cellular localization of neurophysin in the sheep hypothalamus and neurohypophysis. The immunofluorescent technique was used to follow the neurosecretory fibres emanating from the magnocellular nuclei to the lower infundibular stem. It was confirmed that neurophysin was present in both the internal and external zone of the infundibulum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306719
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Neurosecretion in the external and internal zone of the median eminence of the cat and dog (1974)
    Springer
    Cell & tissue research 155 (1974), S. 201-210 
    Details
    ISSN: 1432-0878
    Keywords: External median eminence (Cat, dog) ; Neurosecretory material ; CRF ; Neurophysin ; Immunoperoxidase histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The immunoglobulin-enzyme bridge technique, in association with rabbit antiporcine neurophysin-II has been applied to the median eminence of the dog and cat in order to study the distribution of neurophysin-like antigens throughout this area of the brain and correlate the findings with the corresponding distribution of neurosecretory material (NSM) as revealed by the crotonaldehyde fuchsin stain. Neurophysin and NSM were both present in the hypothalamo-supraoptico-neurohypophysial system—the pathway taken by oxytocin, vasopressin and neurophysin from the hypothalamus to the posterior pituitary lobe. Whereas the tuberoinfundibular tract of the median eminence was almost devoid of NSM, the presence of neurophysin-like material was clearly evident using immunoperoxidase histochemistry. The significance of a protein in the external median eminence possessing determinants cross-reactive against anti-neurophysin serum is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221354
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Time of fixation and the localization of gomori-positive and neurophysin-containing structures in the rat hypothalamus (1975)
    Springer
    Cell & tissue research 162 (1975), S. 523-530 
    Details
    ISSN: 1432-0878
    Keywords: Neurophysin ; Neurosecretory material ; Magnocellular nuclei (rat) ; Postmortem fixation ; Polyacrylamide gel electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rats were decapitated and the complete head stored at 4°C. At various time intervals, up to a maximum of 14 days, the brains and pituitary glands were removed and fixed in formalin-picric acid. Neurosecretory material (NSM), as revealed with crotonaldehyde fuchsin, and neurophysin as demonstrated by means of immunoperoxidase histochemistry were localized in the hypothalamic magnocellular nuclei, median eminence and posterior pituitary gland. NSM and neurophysin were present in the paraventricular (PVN) and supraoptic (SON) nuclei up to periods of 14 days although the cellular morphology was disrupted after about 4 days. After 4 days there was an abundance of positive staining “droplets” in both the SON, PVN and along the nerve fibres of the supraoptico-neurohypophysial tract. Pituitary gland NSM and neurophysin were still present 14 days post mortem. The slab gradient polyacrylamide gel electrophoretic pattern of soluble proteins extracted from the posterior pituitary gland from the experimental and normal animals showed almost complete identity confirming the minimal action of proteolytic enzymes at 4°C up to a period of 6 days. It is concluded that as long as the tissue is cooled, rapid fixation of hypothalamic tissue is not essential for the localization of NSM and neurophysin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209351
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Oxytocinase-immunohistochemical demonstration in the immature and term human placenta (1975)
    Springer
    Cell & tissue research 162 (1975), S. 531-539 
    Details
    ISSN: 1432-0878
    Keywords: Human placenta ; Syncytiotrophoblast ; Oxytocinase ; Anti-oxytocinase serum ; Immunofluorescence histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Oxytocinase (cystine aminopeptidase) was purified from human retroplacental serum by a combination of fractional precipitation, hydroxylapatite chromatography and gel exlusion chromatography on Sephadex G-200. The purified enzyme possessed a specific activity of 980 mIU/mg using L-cystine-di-p-nitroanilide as substrate. This represented a 3200 fold concentration from the starting material in an overall yield of 12%. Antibodies against oxytocinase were raised in rabbits and the γ-globulin fraction labelled with fluorescein isothiocyanate prior to its use in the immunofluorescence histochemical localization of the enzyme in human placental tissue. Oxytocinase was confined to the syncytiotrophoblastic cells of normal term, and immature placentas as well as in placentas from patients suffering from severe toxaemia. Specific immunofluorescence was also present in the outer margins of the chorion and to a lesser extent in the amnion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209352
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Immunocytochemical study of the hypothalamo-neurohypophysial system (1977)
    Springer
    Cell & tissue research 180 (1977), S. 467-490 
    Details
    ISSN: 1432-0878
    Keywords: Oxytocin ; Vasopressin ; Neurophysin ; Magnocellular nuclei ; Neurosecretory pathways ; Osmotic stimulation ; Immunoperoxidase histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Antisera, with cross reactive antibodies removed by affinity chromatography, were used in the immunoperoxidase-bridge technique to study the distribution of oxytocin and vasopressin together with neurophysin in the hypothalamo-neurohypophysial system of the rat. The hormones were demonstrated in different areas of the supraoptic nucleus (SON) and paraventricular nucleus (PVN), in neurosecretory fibres of the hypothalamoneurohypophysial tract, median eminence, and in nerve terminals of the neurohypophysis. Intact normal and rats with hereditary hypothalamic diabetes insipidus (Brattleboro strain), and rats dehydrated by the administration of oral hypertonic saline were studied. In dehydrated rats the hormone concentration in the neurons, and the number of neurons containing hormone varied according to the time of dehydration stress. The observations support the hypotheses that: 1) oxytocin and oxytocinneurophysin, and vasopressin and vasopressin-neurophysin are synthesised in different neurons and are transported along different axons; 2) the SON and PVN are functionally indistinguishable in that neurons containing oxytocin or vasopressin are present in both nuclei; and 3) the two types of neurons respond to osmotic stimulation in a way that is qualitatively the same but quantitatively different.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220169
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    Paper (German National Licenses)
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