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  • 1
    ISSN: 1573-6903
    Keywords: Antibody ; myelin ; cerebroside ; multiple sclerosis ; ELISA ; cerebrospinal fluid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The standard ELISA technique was improved for the detection of antigalactocerebroside antibody in biological fluid. Mouse monoclonal antigalactocerebroside antibody was used to demonstrate specificity and sensitivity of the technique. After optimization of the assay, the usefulness of this measurement for the evaluation of patients with multiple sclerosis was assessed. The presence of antigalactocerebroside antibodies in the cerebrospinal fluid of 20 patients with multiple sclerosis, 10 with other neurological diseases and 10 normal individuals was determined. All the CSF samples from normal individuals were negative. In patients with multiple sclerosis 14 of the 20 samples had elevated levels of antigalactocerebroside antibody, whereas with other neurological diseases 5 out of 10 were positive. Antigalactocerebroside levels were lower in samples from patients during an acute relapse than in those from more chronic cases. These results indicate that the presence of anti-galactocerebroside antibody in cerebrospinal fluid is not specific to MS but may reflect previous damage to myelin.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Clinical autonomic research 3 (1993), S. 101-105 
    ISSN: 1619-1560
    Keywords: Cardioinhibitory ; Carotid sinus syndrome ; Autonomic function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Autonomic reflexes were investigated in patients with the cardioinhibitory carodid sinus syndrome. Heart rate, blood pressure and forearm blood flow responses were recorded during prolonged head-up tilt, the Valsalva manoeuvre, lower body negative pressure and sequential respiratory cycles in ten patients and nine age-matched controls. The mean maximum R—R interval prolongation during carotid sinus massage was 4.4 s. Three patients had syncope during prolonged head-up tilt. Heart rate and blood pressure responses were similar for patients and controls who completed tilt. Responses to lower body negative pressure and Valsalva manoeuvre were similar for both groups. Respiratory sinus arrhythmia was significantly less marked in patients, 7 bpm (0 to 20 bpm) versus 15 bpm (6 to 35 bpm;p = 0.05). Therefore, neck massage and deep breathing produce abnormal cardiac vagal responses, whereas other reflexes, including response to lower body negative pressure and the Valsalva manoeuvre are within the normal range in cardioinhibitory carotid sinus syndrome.
    Type of Medium: Electronic Resource
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