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  • 1
    Electronic Resource
    Electronic Resource
    Type XV collagen exhibits a widespread distribution in human tissues but a distinct localization in basement membrane zones (1996)
    Springer
    Cell & tissue research 286 (1996), S. 493-505 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Collagen ; Type XV ; Basement membrane zones ; Basement membrane ; Immunohistochemistry ; Antibody ; Recombinant protein ; Human tissues
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The collagen family of proteins consists of 19 types encoded by 33 genes. One of the more recently discovered collagens is the α1 chain of type XV. Type XV collagen is comprised of a 577-amino-acid, highly interrupted, triple-helical region that is flanked by amino and carboxy noncollagenous domains of 555 and 256 residues, respectively. To address questions of where this collagen is localized and what its function may entail, we produced a bacteria-expressed recombinant protein representing the first half of the type XV collagen carboxy-terminal domain in order to generate highly specific polyclonal antisera. Immunoscreening of an expression library with the affinity-purified antibody revealed three clones coding for part of the type XV triple-helical region and the entire noncollagenous carboxy-terminus. Western blot analysis of human tissue homogenates identified a 116-kDa collagenase-sensitive protein and a 27-kDa collagenase-resistant fragment, whose electrophoretic mobilities were unchanged in the presence and absence of reductant. Northern blot hybridization to human tissue RNAs indicated that type XV has a prevalent and widespread distribution. To determine the precise localization of type XV collagen, immunohistochemical analyses at the light- and electron-microscopic levels were performed. Type XV exhibited a surprisingly restricted and uniform presence in many human tissues as evidenced by a strong association with vascular, neuronal, mesenchymal, and some epithelial basement membrane zones. These data suggest that type XV collagen may function in some manner to adhere basement membrane to the underlying connective tissue stroma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050719
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  • 2
    Electronic Resource
    Electronic Resource
    Lymphotaxis induced by ultraviolet microbeam irradiation of fibroblasts in tissues cultured from mouse embryosThis investigation was supported in part by a PHS Research Grant GM-09397, from the Division of General Medical Sciences, U. S. Public Health Services, and in part by Institutional Grants from the American Cancer Society. (1967)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 159 (1967), S. 199-209 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: When portions of the cytoplasm of fibroblasts cultured from mouse embryo tissues are irradiated with heterochromatic microbeams of ultraviolet light, lymphocytes are attracted (Lymphotaxis) to the injured areas. The lymphocytes approach in the “hand mirror” shape characteristic of tissue cultures. On reaching the fibroblast, pseudopodia shoot out to “lick” the wound and then the lymphocyte “worms” its way across the cell. Only one lymphocyte contacts a fibroblast but the same one may make several passes. It is postulated that lymphocytes are cleaning the wounded area of undesirable irradiation products and/or applying healing substances to the wounded fibroblast.Irradiation of lymphocytes in portions of the cytoplasm fails to evoke responses from nearlby lymphocytes. Instead, the cytoplasm whirls around the nucleus as if the irradiation products can not leave the cell, resulting in convulsions and death.A technique is described by which relatively pure cultures of lymphocytes may be obtained from leukocyte cultures by selectively killing all granular leukocytes with excessive exposure to microbeams of ultraviolet light. Lymphocytes are harvested and introduced into fibroblast cultures to study experimental lymphotaxis.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091590209
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  • 3
    Electronic Resource
    Electronic Resource
    Fusion of nucleoli in cells cultured from the heart of Triturus viridescensThis study was started at The University of Chicago (supported by A.E.C., Office of Naval Research, and the Abbott Research Fund). Continued support by the Institutional Grant to The Hahnemann Medical College by the American Cancer Society (C-44, C-90) is gratefully acknowledged. (1961)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 139 (1961), S. 155-165 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091390207
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  • 4
    Electronic Resource
    Electronic Resource
    The effects of ultraviolet microbeam irradiation on the eosinophil granular leukocytes of Triturus viridescensSupported by the American Cancer Society Institutional grant to the Hahnemann Medical College (C-124). (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 142 (1962), S. 81-87 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091420112
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  • 5
    Electronic Resource
    Electronic Resource
    Ultraviolet microbeam irradiation of vesicle-like bodies in agranular interphase cells cultured from Triturus viridescensThis investigation was supported by a PHS Research Grant no. GM-09397, from the Division of General Medical Sciences, U. S. Public Health Services. (1963)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 147 (1963), S. 423-429 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In flattened cells cultured from the heart of Triturus viridescens, cytoplasmic vesicles possessing a decreased refractive index were observed in the vicinity of the nucleus. The vesicles displayed a bubbling phenomenon when viewed by dark medium phase contrast microscopy and recorded by time lapse photography. Usual fat stains failed to reveal the vesicles in fixed preparations. DaFano's and Aoyama's methods employing silver imparted a brown-yellow color to the vesicles, but because the cell nucleus also stained the same color, no identification could be advanced.Heterochromatic microbeams of ultraviolet light focused into the vesicular complex caused swelling and fusion of the vesicles, followed by regression in size until they could no longer be seen. Cytoplasmic irradiation adjacent to and at relatively great distances from the vesicles failed to evoke this reaction from the vesicles. An interesting side effect was recorded in the nucleus: a diminished granularity of the chromatin. This effect was likened to previously recorded cytoplasmic irradiation effects; that of nuclear pyknosis in eosinophils, and spindle destruction in early mitotic cells. Irradiation of fat droplets caused none of these effects. Further studies are aimed at defining the nature of these vesicles, with emphasis on the vesicles being pinocytotic vesicles, endoplasmic reticulum or Golgi vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091470314
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  • 6
    Electronic Resource
    Electronic Resource
    An interferometric analysis of nucleoli in cultured mesothelial cells (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 201 (1981), S. 13-21 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Previous studies have indicated that total nucleolar area and volume remain constant regardless of the number of nucleoli. The question remains whether this relationship is valid for mass. To answer this, total nucleolar mass values were obtained from nuclei of living mesothelial cells in culture possessing one to four nucleoli. The nucleolar mass was calculated using interferometry. The mean total nucleolar dry mass for cells with one, two, three, and four nucleoli was 40 × 10-12gm, 38.4 × 10-12gm, 39.1 × 10-12gm, and 41.4 × 10-12gm, respectively. These data suggest that on the average, each cell had approximately the same total nucleolar dry mass regardless of the number of nucleoli.In an additional study, interferometry was employed to reveal changes in nucleolar mass and concentration during a seven-hour period. It was concluded that the nucleolus is a dynamic organelle, with its total mass varying in time from an average 40 × 10-12gm with a mean concentration of 22.2gm/100cm3.
    Additional Material: 40 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092010103
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  • 7
    Electronic Resource
    Electronic Resource
    Properties and purification of a colony-stimulating factor of granulocytes and macrophages produced by mouse spleen cells (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 180 (1987), S. 178-184 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Mouse splenocytes are induced by pokeweed mitogen to secrete a factor that stimulates mouse hemopoetic (spelling per Nomina Histologica in the Nomina Anatomica, 5th edition, 1983, Williams and Wilkins, Baltimore) progenitor cells to undergo proliferation and differentiation into granulocytes and macrophages in a semi-solid culture system. The granulocyte and macrophage colony-stimulating factor (GM-CSF) was purified with a four-step procedure that includes ultrafiltration, chromatography on DEAE-agarose, Sephacryl S-200, and chromatofocusing gel. The isoelectric point (pI) of 4.2 of the GM-CSF was determined by analytical isoelectrofocusing gel electrophoresis. The sensitivity of the biological activity of GM-CSF to digestion by trypsin and neuraminidase suggests that GM-CSF is a glycoprotein with its sugar moieties at the active site. The GM-CSF is also sensitive to heat denaturation at 60°C or higher suggesting that a three-dimensional conformation is required for its biological activity. The molecular w eight of GM-CSF is approximately 57,000 Daltons as determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001800207
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