ISSN:
1432-072X
Keywords:
Elongation factor Tu
;
Microcomplement fixation
;
Western blot
;
Cross-reactivity
;
Antibody binding site
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Polyclonal antibodies were prepared against the purified elongation factor Tu (EF-Tu) of Escherichia coli and Bacillus subtilis. Using the methods of Western blotting and microcomplement fixation the cross-reactivities of EF-Tu of 19 different prokaryotes were determined. The immunological distance were compared with the results of 16S rRNA oligonucleotide analysis. An unexpectedly high cross-reactivity was revealed between the EF-Tu of B. subtilis and the antiserum against the EF-Tu of E. coli. A comparison of the predicted amino acid sequences from the tuf-genes of E. coli and B. subtilis yielded two identical peptide fragments that are likely candidates for antibody binding sites.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00409660
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