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  • Antibody production  (1)
  • California  (1)
  • Cytochrome P-450  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Immunological characterization of constitutive isozymes of cytochrome P-450 from rainbow trout. Evidence for homology with phenobarbital-induced rat P-450s
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular 1037 (1990), S. 155-160 
    Details
    ISSN: 0167-4838
    Keywords: (Rainbow trout) ; (Rat) ; Cytochrome P-450 ; Immunochemical homology
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0167-4838(90)90161-8
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Lily cofactor-independent phosphoglycerate mutase: purification, partial sequencing, and immunolocalization (1996)
    Springer
    Planta 200 (1996), S. 343-352 
    Details
    ISSN: 1432-2048
    Keywords: Antibody production ; Enzyme immunolocalization ; Lilium ; Phosphoglycerate mutase (cofactor independent) ; Vitronectin antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cofactor-independent phosphoglycerate mutase (PGAM-i) was isolated to homogeneity from monocotyledonous Lilium longiflorum Thunb. Two-dimensional (2D) polyacrylamide gel electrophoresis resolved three PGAM-i forms. This enzyme was originally identified by cross-reactivity to antibodies affinity-purified from 2D gels using human vitronectin (VN). Antibody produced against a denatured protein spot from a 2D gel did not recognize VN protein, but partial protein and DNA sequencing showed similarity of the former protein to maize PGAM-i. Immunoblots from roots, styles, leaves, and anthers showed the presence of PGAM-i in all tissues examined; it was isolated predominantly from the soluble cell fraction, with some present in the insoluble cell fraction. Immunoelectron microscopy demonstrated its localization in the cytoplasm and plastids in root cells near the apical meristem. In addition, immunogold labeling detected signals from the nucleus. The immunohistochemical localization of the enzyme in the nucleus, as well as in the cytosol and plastids, indicates that lily PGAM-i might have multiple functions in the cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00200302
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A change-point model for reporting delays under change of AIDS case definition (2000)
    Springer
    European journal of epidemiology 16 (2000), S. 1135-1141 
    Details
    ISSN: 1573-7284
    Keywords: California ; Case definition ; Change-point ; Disease surveillance ; Incidence ; Reporting delays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Accurate monitoring of disease incidence is of major public health concern. The time delay between diagnosis and the date of reporting creates bias in estimating disease incidence. Changes in case definition are expected to have an impact on the time lag of case reporting. We propose a change-point model for reporting delays in AIDS that takes into account recent changes in the AIDS definition in US and European countries. The model was applied to California AIDS surveillance data and the distribution of reporting delays before and after the recent change of definition in 1993 were analyzed in terms of contributing factors. The overall significance of the model with change-point as compared to the model without change-point indicates that the effect of the 1993 change in definition on the distribution of reporting delays was highly significant (p 〈 0.0001). Overall, reporting delay of cases initially diagnosed with AIDS-defining diseases before 1993 was shorter compared to after 1993; reporting delay of cases initially diagnosed meeting the 1993 immunologic case definition was shorter than of those initially diagnosed with AIDS-defining diseases. Region of residence, mode of exposure, race/ethnicity and time of diagnosis emerged as the main covariates in the models. The method introduced here applies to current and possible future changes of the AIDS case definition as well as changes in diagnostic criteria or case definition in diseases other than AIDS. We demonstrate that such changes may be accompanied by sizeable changes in the distribution of reporting delays, and thus adjustment for reporting delays must be recalibrated after a change in definition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1010955827954
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    Paper (German National Licenses)
    Fulltext
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