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  • 1
    Electronic Resource
    Electronic Resource
    The nature of callose produced during self-pollination inSecale cereale (1980)
    Springer
    Planta 148 (1980), S. 498-509 
    Details
    ISSN: 1432-2048
    Keywords: Callose ; Pollination ; Secale ; Self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The response to incompatible (self) pollination in rye (Secale cereale L.) includes the rapid deposition in the germinating pollen grain and pollen tube of a substance that stains with aniline blue, resorcin blue and calcofluor, and in these respects resembles callose. This substance has been isolated and analysed by acid hydrolysis and methylation as well as specific enzyme hydrolysis. It contains a glucan component with 1,4-β-glucosidic and 1,3-β-glucosidic linkages within the same linear chains. The proportion of 1,4-to 1,3-glucosidic linkages in the preparation is 77∶9.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02395321
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Composition of the cell walls of Nicotiana alata Link et Otto pollen tubes (1985)
    Springer
    Planta 166 (1985), S. 128-133 
    Details
    ISSN: 1432-2048
    Keywords: Arabinan ; Callose ; Cell wall (pollen) ; Nicotiana (Pollen) ; Pollen tube (wall)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell walls isolated from pollen of Nicotiana alata germinated in vitro contain glucose and arabinose as the predominant monosaccharides. Methylation analysis and cytochemical studies are consistent with the major polysaccharides being a (1→3)-β-D-glucan (callose) and an arabinan together with small amounts of cellulose. The cell walls contain 2.8% uronic acids. Alcian blue stains the pollen-tube walls intensely at the tip, indicating that acidic polysaccharides are concentrated in the tip. Synthetic aniline-blue fluorochrome is specific primarily for (1→3)-β-D-glucans and stains the pollen-tube walls, except at the tip. Protein (1.5%), containing hydroxyproline (2.4%), is present in the cell wall.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397395
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The nature of callose produced during self-pollination inSecale cereale (1980)
    Springer
    Planta 148 (1980), S. 498-509 
    Details
    ISSN: 1432-2048
    Keywords: Callose ; Pollination ; Secale ; Self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The response to incompatible (self) pollination in rye (Secale cereale L.) includes the rapid deposition in the germinating pollen grain and pollen tube of a substance that stains with aniline blue, resorcin blue and calcofluor, and in these respects resembles callose. This substance has been isolated and analysed by acid hydrolysis and methylation as well as specific enzyme hydrolysis. It contains a glucan component with 1,4-β-glucosidic and 1,3-β-glucosidic linkages within the same linear chains. The proportion of 1,4-to 1,3-glucosidic linkages in the preparation is 77∶9.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00552666
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Isolation and partial characterization of components of Prunus avium L. styles, including an antigenic glycoprotein associated with a self-incompatibility genotype (1982)
    Springer
    Planta 156 (1982), S. 505-516 
    Details
    ISSN: 1432-2048
    Keywords: Antigen ; Glycoprotein ; Pollination ; Prunus ; S-allele ; Self-incompatibility ; Style mucilage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several components of buffer extracts of Prunus avium L. styles (cv. Lambert, S 3 S 4) have been isolated and partially characterized: the major component is a glycoprotein (molecular weight approx. 90,000; 95% protein, 5.4% carbohydrate). A “sticky” uronic-acid-containing component and an arabinogalactan are also present. Two minor components are an antigenic glycoprotein associated with the self-incompatibility genotype (Antigen S) and a component found in styles of all Prunus species (Antigen P). The isolated glycoproteins have a substantial carbohydrate content (Antigen P 17.2%; Antigen S 16.3%), and have apparent molecular weights of 32,000 (Antigen P) and 37,000–39,000 (Antigen S). They are antigenically quite distinct. Material corresponding to Antigen S is secreted into the medium of suspension-cultured callus cells raised from both leaf and stem of P. avium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392772
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    The location of (1→3)-β-glucans in the walls of pollen tubes of Nicotiana alata using a (1→3)-β-glucan-specific monoclonal antibody (1991)
    Springer
    Planta 185 (1991), S. 1-8 
    Details
    ISSN: 1432-2048
    Keywords: Callose ; Cell wall ; (1→3)-β-Glucan ; Nicotiana alata ; Pollen tube ; Monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The location of the (1→3)-β-glucan, callose, in the walls of pollen tubes in the style of Nicotiana alata Link et Otto was studied using specific monoclonal antibodies. The antibodies were raised against a laminarinhaemocyanin conjugate. One antibody selected for further characterization was specific for (1→3)-β-glucans and showed no binding activity against either a cellopentaose-bovine serum albumin (BSA) conjugate or a (1→3, 1→4)-β-glucan-BSA conjugate. Binding was inhibited by (1→3)-β-oligoglucosides (DP, 3–6) with maximum competition being shown by laminaripentaose and laminarihexaose, indicating that the epitope included at least five (1→3)-β-linked glucopyranose residues. The monoclonal antibody was determined to have an affinity constant for laminarihexaose of 2.7. 104M−1. When used with a second-stage gold-labelled, rabbit anti-mouse antibody, the monoclonal antibody probe specifically located the (1→3)-β-glucan in the inner wall layer of thin sections of the N. alata pollen tubes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194507
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Tissue-specific antigens secreted by suspension-cultured callus cells of Prunus avium L. (1981)
    Springer
    Planta 153 (1981), S. 115-124 
    Details
    ISSN: 1432-2048
    Keywords: Antigen ; Callus ; Cell suspension culture ; Prunus ; Rosaceae ; Secretions, extracellular ; Self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A number of antigenic components are secreted into the medium by P. avium callus cells derived from different tissues and grown in suspension culture. These antigens have been detected using antiserum raised in rabbits to a protein fraction secreted by P. avium leaf callus. One antigen is specific to leaf tissue and is secreted by callus cells derived from stem, pistil and anthers as well as leaves. A second antigen is, in intact organs, restricted to styles of a particular self-incompatibility (S) genotype, but is also secreted by callus cells derived from the leaf. Another antigen, apparently not organ-specific, is secreted by all calli tested, including Rosa (cv. Paul's Scarlet).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384092
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    The application of Sirofluor, a chemically defined fluorochrome from aniline blue for the histochemical detection of callose (1984)
    Springer
    Protoplasma 122 (1984), S. 191-195 
    Details
    ISSN: 1615-6102
    Keywords: Aniline blue ; Callose ; Cell walls ; Fluorochrome ; (1 → 3)-β-glucan ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sirofluor, a chemically defined fluorochrome from aniline blue in aqueous unbuffered solutions, complexes with isolated (1 → 3)-β-glucans, but not (1 → 4)-β-glucans, after embedding in JB-4 resin and sectioning. Under these conditions, callose deposits in plant tissues give a brilliant yellow fluorescence with essentially no background fluorescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281696
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    Paper (German National Licenses)
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