Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Observations on enzymatically isolated, living and fixed embryo sacs in several angiosperm species (1985)
    Springer
    Planta 165 (1985), S. 225-231 
    Details
    ISSN: 1432-2048
    Keywords: Antirrhinum (embryo-sac isolation) ; Embryo sac (isolated) ; Helianthus (embryo-sac isolation) ; Nicotiana (embryo-sac isolation)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A technique has been developed for isolating embryo sacs (ESs) by enzymatic maceration. Ovules were macerated in a mixture of pectinase, cellulase and, in some cases, snailase and pectolyase Y-23. The ovular tissues were removed and the ESs were isolated in toto. Embryo sacs were isolated from both fixed and fresh ovules of Antirrhinum majus L., Helianthus annuus L. and Nicotiana tabacum L. Fluorochromasia by fluorescein diacetate showed that the ESs isolated from fresh ovules were viable. The method has promise for various histochemical and cell-physiological studies and quite possibly also for in-vitro culture of ESs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395045
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    In vitro induction of haploid plants from unpollinated ovaries and ovules (1982)
    Springer
    Theoretical and applied genetics 63 (1982), S. 97-104 
    Details
    ISSN: 1432-2242
    Keywords: Haploid induction ; Ovary culture ; Ovule culture ; Gynogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A recent advance in plant tissue culture and experimental embryology is the successful induction of haploid plants by the culture of unpollinated ovaries or ovules. It means that not only the microspore but also the megaspore or female gametophyte of angiosperms can be triggered in vitro to sporophytic development, thus opening a new way to genetical research and haploid breeding. Since data so far accumulated are attractive, though not so rich, this paper tends to offer a preliminary review on this problem, covering the historical description, culture experiments, embryological observations, cytological and genetical studies and a brief discussion on its perspectives.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303687
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...