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  • Umbilical blood vessels  (2)
  • Aortic endothelial cells  (1)
  • Arginine-vasopressin  (1)
  • Localization  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Peptides 12 (1991), S. 1095-1103 
    ISSN: 0196-9781
    Keywords: Aortic endothelial cells ; Arginine-vasopressin ; Colocalization ; Endothelin-1 ; Localization ; Primary culture ; Serotonin
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0878
    Keywords: Neuropeptide Y ; Atrial natriuretic peptide ; Endothelial cells ; Umbilical blood vessels ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The localization of neuropeptide Y (NPY) and atrial natriuretic peptide (ANP) in the endothelial cells of human umbilical blood vessels was studied using the pre-embedding peroxidase-antiperoxidase (PAP) technique for electron microscopy and avidin-biotin-complex (ABC) immunostaining for endothelial cells cultured from umbilical vein. Subpopulations of NPY- and ANP-immunoreactive endothelial cells were present in term umbilical vein and artery. The umbilical vein contained more positive cells than the artery. The percentage of NPY- and ANP-immunoreactive umbilical vein cells in culture was 32% and 44%, respectively, out of a total of 3013 cells examined. The possibility that these potent vasoactive substances located in the endothelial cells of the non-innervated umbilical vessels are involved in the local regulation of blood flow is discussed.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0878
    Keywords: Umbilical blood vessels ; Endothelial cells ; Vasoactive substances ; Colocalization ; Immunogold labelling ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Human umbilical vessels are unique in lacking any innervation; thus endothelial cells may play the major role in local control and regulation of the blood flow. In the present study, we examined ultrathin sections of cultured human umbilical vein endothelial cells and tissue preparations of umbilical vein and artery, immunostained by the post-embedding colloidal gold double-labelling technique. We observed colocalization of atrial natriuretic peptide and neuropeptide Y, as well as colocalization of atrial natriuretic peptide and neuropeptide Y with other vasoactive substances, namely, vasoactive intestinal peptide, substance P, calcitonin gene-related peptide and arginine vasopressin. The functional significance of the colocalization of these vasoactive substances in the human umbilical vessel endothelial cells is discussed.
    Type of Medium: Electronic Resource
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