ISSN:
1573-6830
Keywords:
Ca-binding proteins
;
parvalbumin
;
calbindin D-28K
;
sarcoplasmic calcium-binding protein
;
electrical activity
;
mollusk neurons
;
Aplysia californica
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary 1. Calcium (Ca)-binding proteins of neuronal ganglia and of single, identified neurons of the marine mollusk,Aplysia californica, were investigated. Using transblot/45Ca overlays two proteins, atM r 45,000 andM r 23,000, with a high Ca-binding ability were found. 2. Western blot analysis revealed that the protein atM r 45,000 could be separated by 2D-PAGE into proteins withM r 40,000 andM r 43,000. The protein atM r 40,000 immunocross-reacted with antisera directed against parvalbumin and rat calbindin D-28K, indicating a novel Ca-binding protein sharing common antigenic determinants for both proteins. 3. The protein atM r 23,000 could be separated into a group of proteins withM r 13,000–20,000 which showed a high degree of similiarity to sarcoplasmatic calcium-binding proteins (SCP). 4. We further investigated the protein pattern of single, identified neurons of different electrical activity (bursting, beating, and silent) by 2D-PAGE. Major differences were found in the range of lowM r and lowpI, where Ca-binding proteins are generally located. A protein at high concentrations characteristic for silent cells migrated at a position similar to crayfish SCP. 5. The results show that various Ca-binding proteins are characteristic for neurons in theAplysia nervous system and support the idea that they may effect the electrical behavior of nerve cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00711419
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